Behavioral, biochemical and histopathological toxic profiles induced by sub-chronic cannabimimetic WIN55, 212–2 administration in mice

Table 1 Proteomic analysis of brains’ glutamate and GABA levels of WIN55, 212–2 treated mice

Gender	Control Mean ± SD	Vehicle Mean ± SD	WIN55, 212–2 (0.05 mg/kg) Mean ± SD	WIN55, 212–2 (0.1 mg/kg) Mean ± SD
Male mice
Glutamate	0.072 ± 0.013 ^bcd**	0.095 ± 0.005^{a,c,d**}	0.126 ± 0.022^{a* b* d***}	0.272 ± 0.010 ^a*bc**
GABA	0.430 ± 0.003 ^b*cd**	1.60 ± 0.001^{a*,c,d**}	7.40 ± 0.230^{a*b* d***}	10.1 ± 0.79 ^a*bc**
Female mice
Glutamate	0.59 ± 0.097^{bNS c*d*}	0.56 ± 0.097 ^{a NS c* d*}	5.41 ± 4.65 ^{a b* d***}	11.59 ± 4.11^{a* b* c***}
GABA	9.25 ± 0.594^{bNS c*d*}	9.86 ± 0.878^{aNS,c*, d*}	12.7 ± 1.17 ^{a*.b,d**}	23.8 ± 2.21 ^{a*,b,c**}

One-way ANOVA test was undertaken for each mice sex. Data expressed as mean ± SD
NS: non-significant
^*p < 0.05
^**p < 0.01
^***p < 0.001
^acomparison with the control group
^bcomparison with the vehicle group
^ccomparison with the low dose group
^dcomparison with high dose group

ISSN: 2050-6511

Gender	Control Mean ± SD	Vehicle Mean ± SD	WIN55, 212–2 (0.05 mg/kg) Mean ± SD	WIN55, 212–2 (0.1 mg/kg) Mean ± SD
Male mice
Glutamate	0.072 ± 0.013 ^bcd**	0.095 ± 0.005^{a,c,d**}	0.126 ± 0.022^{a* b* d***}	0.272 ± 0.010 ^a*bc**
GABA	0.430 ± 0.003 ^b*cd**	1.60 ± 0.001^{a*,c,d**}	7.40 ± 0.230^{a*b* d***}	10.1 ± 0.79 ^a*bc**
Female mice
Glutamate	0.59 ± 0.097^{bNS c*d*}	0.56 ± 0.097 ^{a NS c* d*}	5.41 ± 4.65 ^{a b* d***}	11.59 ± 4.11^{a* b* c***}
GABA	9.25 ± 0.594^{bNS c*d*}	9.86 ± 0.878^{aNS,c*, d*}	12.7 ± 1.17 ^{a*.b,d**}	23.8 ± 2.21 ^{a*,b,c**}