Table 1 Sequences of the primer pairs used for RT-qPCR
Mouse | ||
|---|---|---|
Primer | Sequence 5’→3’ | GenBank accession number |
IFN-γ | F: GCCACGGCACAGTCATTGA R: TGCTGATGGCCTGATTGTCTT | NM_008337.4 |
IL-1β | F: GGACCTTCCAGGATGAGGAC R: GTTCATCTCGGAGCCTGTAG | NM_008361.4 |
IL-4 | F: ATCATCGGCATTTTGAACGAGGTC R: ACCTTGGAAGCCCTACAGACGA | NM_021283.2 |
IL-5 | F: GAAGTGTGGCGAGGAGAGAC R: GCACAGTTTTGTGGGGTTTT | NM_010558.1 |
IL-12Â A | F: GATGACATGGTGAAGACGGC R: AGGCACAGGGTCATCATCAA | NM_008351.3 |
IL-13 | F: GGGACATGGTTTGCTGCCTA R: AGACAGGAGTGTTGCTCTGG | NM_008355.3 |
GAPDH | F: TGCTCCTCCCTGTTCCAGA R: TACGGCCAAATCCGTTCACA | NM_008084.3 |
Human | ||
Primer | Sequence 5’→3’ | GenBank accession number |
CCL17 | F: ACAAGGGGATGGGATCTCCCTCAC R: ACTGCTCCAGGGATGCCATCGTTT | NM_002987.3 |
CCL22 | F: AGGACAGAGCATGGATCGCCTACA R: TAATGGCAGGGAGGTAGGGCTCCT | NM_002990.5 |
IL-1β | F: GCTGATGGCCCTAAACAGATGAA R: TGAAGCCCTTGCTGTAGTGGTG | NM_000576.3 |
IL-6 | F: CCCCTGACCCAACCACAAAT R: CATTTGCCGAAGAGCCCTCA | NM_000600.5 |
GAPDH | F: AGACACCATGGGGAAGGTGA R: TGGAATTTGCCATGGGTGGA | NM_002046.7 |