Fig. 2

BDMC administration inhibits inflammatory responses in ALI mice. Mice were intragastrically administered with BDMC (100 mg/kg/day) for 3 days, and then intratracheally injected with a single dose of LPS (5 mg/kg). Mice were sacrificed 12 h post-LPS injection with the lungs collected for further examination. (A-C) The levels of inflammatory cytokines were assessed in murine lungs (n = 6). (D-E) The levels of inflammatory cytokines were assessed in BALF (n = 6). (F-H) The infiltrations of total cells, macrophages and neutrophils in BALF (n = 6). (I) The MPO activity in lung tissues (n = 6). (J-L) The western blot images and quantitative data of p65 phosphorylation and nuclear translocation (n = 6). Values represent the mean ± SD. *P<0.05 versus Saline + Vehicle; ^#P<0.05 versus LPS + Vehicle