Fig. 4

BDMC treatment inhibits LPS-induced inflammation and oxidative stress in macrophages. Primary mouse peritoneal macrophages were isolated and pretreated with BDMC (10 µmol) for 12 h prior to LPS insult (100 ng/mL). (A-C) The levels of inflammatory cytokines in cell medium (n = 6). (D) NF-κB activity in macrophages (n = 6). (E-G) The western blot images and quantitative data of p65 phosphorylation and nuclear translocation (n = 6). (H) The ROS generation in macrophages were assessed by a DCFH-DA probe (n = 6). (I-J) The levels MDA and GSH in macrophages (n = 6). (K) The levels of total SOD activity in macrophages (n = 6). Values represent the mean ± SD. *P<0.05 versus PBS + Vehicle; ^#P<0.05 versus LPS + Vehicle