Fig. 5

BDMC treatment protects against LPS-induced ALI via activating AMPKα. Mice were intragastrically administered with BDMC (100 mg/kg/day) for 3 days, and then intratracheally injected with a single dose of LPS (5 mg/kg). Mice were sacrificed 12 h post-LPS injection with the lungs collected for further examination. (A) The western blot images and quantitative data about AMPKα phosphorylation were presented (n = 6). For AMPKα inhibition, mice were intraperitoneally treated with CpC (20 mg/kg) once two days for 3 times before BDMC administration. (B-D) The levels of IL-1β, IL-6 and TNF-α in lung tissues (n = 6). (E-H) The levels of ROS, MDA, GSH and total SOD activity were detected in lung tissues (n = 6). (I-J) The pulmonary function as determined by airway resistance and pulmonary ventilation (n = 6). (K-L) The arterial blood gas analysis of PaO₂ and PaCO₂ (n = 6). (M) The total proteins were determined in BALF (n = 6). (N) The LDH activity in lung tissues (n = 6). Values represent the mean ± SD. *P < 0.05 versus the matched group. In Fig. 5A, *P < 0.05 versus Saline + Vehicle, ^#P < 0.05 versus LPS + Vehicle