Fig. 3

Representative immunofluorescence images of (A) Claudin-2 and (B) Occludin immunoreactivity (green) from CHOW, MC, MC + AC and AC only groups. Morphometric analysis (calculated as %ROI) of (C) Claudin-2, and (D) Occludin immunoreactivity where Y-axis represents % positive immunoreactive area (% ROI) (n = 3; mean value taken from three separate microscopic fields). Data were represented as mean ± SEM and statistical significance was tested using unpaired t-test between the two groups (ns – non significant, * p < 0.05, ** p < 0.01, *** p < 0.001), followed by Bonferroni Dunn Post hoc corrections. Correlation plot between intestinal acetate concentration and intestinal (E) Claudin-2, and (F) Occludin levels