Fig. 4

Representative immunohistochemistry images of (A) CD68 and (B) α-SMA immunoreactivity from CHOW, MC, MC + AC and AC only groups. Morphometric analysis (calculated as %ROI) of (C) CD68, and (D) α-SMA immunoreactivity where Y-axis represents % positive immunoreactive area (% ROI) (n = 3; mean value taken from three separate microscopic fields). Data were represented as mean ± SEM and statistical significance was tested using unpaired t-test between the two groups (ns – non significant, * p < 0.05, ** p < 0.01, *** p < 0.001), followed by Bonferroni Dunn Post hoc corrections. Correlation plot between serum acetate concentration and liver (E) CD68, and (F) α-SMA levels