Table 1 Sequence of Primers used for real-time PCR
Genes | Gene description | Accession number | Primer Sequence |
|---|---|---|---|
Nrf 2 | Nuclear factor, erythroid 2-like 2 | NC_005102.4 | F: -5′-GGCCCTCAATAGTGCTCAG‐3′ R: -5′-TAGGCACCTGTGGCAGATTC‐3′ |
SOD | Super oxide dismutase | NM_017050 | F: 5′- -GCAGAAGGCAAGCGGTGAAC‐3′ R:5′‐ TAGCAGGACAGCAGATGAGT-3′ |
BRCA | Breast cancer gene 1 | AF036760.1 | F: 5′- CCCCTGATCCCGATAATGCT‐3′ R: 5′- TGTGAAGGGCTGCTCTTGTA ‐3′ |
TNF-α | Tumor necrosis factor alpha | NM_012675.3 | F: 5′-ACACACGAGACGCTGAAGTA-3′ R: 5′-GGAACAGTCTGGGAAGCTCT-3′ |
IL-1β | Interleukin-1 beta | NM_031512.2 | F: 5′- TTGAGTCTGCACAGTTCCCC ‐3′ R: 5′- GTCCTGGGGAAGGCATTAGG ‐3′ |
VEGF | Vascular endothelial growth factor A | NM_001287110.1 | F: 5′- TTCCTGTAGACACACCCACC ‐3′ R: 5′- TCCTCCCAACTCAAGTCCAC ‐3′ |
AKT | AKT Serine/Threonine Kinase 1 | NM_033230.3 | F: 5′- CTGCCCTTCTACAACCAGGA‐3′ R: 5′- GTGCTGCATGATCTCCTTGG ‐3′ |
mTOR | Mechanistic target of Rapamycin Kinase | NM_019906.2 | F: 5′- TCTGCACTTGTTGTTGCCTC‐3′ R: 5′- ACAATCGGGTGAATGATGCG ‐3′ |
PIk3ca | Phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha | NM_133399.3 | F: 5′- TAGTGTCCGGGAAAATGGCT ‐3′ R: 5′- GGCATGCTCTTCGATCACAG ‐3′ |
GAPDH | Glyceraldehyde3-phosphate dehydrogenase | NC_005103.4 | F: − 5′-ACCACAGTCCATGCCATCAC-3′ R: − 5′-TCCACCACCCTGTTGCTGTA-3′ |