Table 1 The primers sequences for genes
From: Effects of nicotine on microRNA-124 expression in bile duct ligation-induced liver fibrosis in rats
Genes | Sequences | Accession number | |
|---|---|---|---|
rno-miR-124-5p | Target sequence* | 5´ CGUGUUCACAGCGGACCUUGAU 3´ | MIMAT0004728 |
rno-miR-191a-5p | Target sequence* | 5´ CAACGGAAUCCCAAAAGCAGCUG 3´ | MIMAT0000866 |
CCL-2 | Forward | 5´ GGGCCTGTTGTTCACAGTTGC 3´ | NM_031530.1 |
Reverse | 5´ GGGACACCTGCTGCTGGTGAT 3´ | ||
STAT-3 | Forward | 5ˊ-TGGAAGAGGCGGCAGCAGATAGC-3 ́ | X91810.1 |
Reverse | 5ˊ-GCACGGCCCCCATTCCCACAT- 3 ́ | ||
α-SMA | Forward | 5´ AACACGGCATCATCACCAAC 3´ | NM_031004.2 |
Reverse | 5´ CACAGCCTGAATAGCCACATAC 3´ | ||
β-actin | Forward | 5´ TCACCCACACTGTGCCCCATCTACGA 3´ | NM_031144.3 |
Reverse | 5´ CAGCGGAACCGCTCATTGCCAATGG 3´ | ||