Fig. 3

Calycosin decreased macrophage M2 polarization in OVA-induced asthmatic mice. (A) The levels of M2 macrophage markers arginase 1 (ARG1) and (B) IL-10 in the BALF and (C) chitinase-like 3 (YM1) and (D) Mannose receptor C-type 1 (MRC1) in the lung tissues were estimated by ELISA and RT-qPCR, respectively. (E) IF was performed to present co-localization of MRC1 and EGF-like module-containing mucin-like hormone receptor-like 1 (F4/80) in the lung tissues. Scale bar = 50 μm. n = 6 for each group. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, compared with the sham group; ^#P < 0.05, ^###P < 0.001, compared with the OVA group. “Ca” represents calycosin