Volume 14 Supplement 1

6th International Conference on cGMP: Generators, Effectors and Therapeutic Implications

Open Access

Evidence for functional coupling of cGMP/cGKI signalling and TRPC channels in endothelium but not in vascular smooth muscle

  • Florian Loga1Email author,
  • Katrin Domes1,
  • Marc Freichel2, 3,
  • Veit Flockerzi3,
  • Alexander Dietrich4,
  • Lutz Birnbaumer5,
  • Franz Hofmann1 and
  • Jörg W Wegener1
BMC Pharmacology and Toxicology201314(Suppl 1):P40

https://doi.org/10.1186/2050-6511-14-S1-P40

Published: 29 August 2013

Background

Signaling via cGMP-dependent protein kinase I (cGKI) is the major pathway in vascular smooth muscle (SM), by which endothelial NO regulates vascular tone. Recent evidence suggests that canonical transient receptor potential (TRPC) channels are targets of cGKI in SM and mediate the relaxant effects of cGMP signaling. We tested this concept by investigating the role of cGMP/cGKI signaling on vascular tone and peripheral resistance using Trpc6-/-, Trpc3-/-, Trpc3-/-/6-/-, Trpc1-/-/3-/-/6-/-, and SM-specific cGKI-/- (sm-cGKI-/-) mice.

Results

α-adrenergic stimulation induced similar contractions in L-NAME-treated aorta and comparably increased peripheral pressure in hind limbs from all mouse lines investigated. After α-adrenergic stimulation, 8-Br-cGMP diminished similarly aortic tone and peripheral pressure in control, Trpc6-/-, Trpc3-/-, Trpc3-/-/6-/-, and Trpc1-/-/3-/-/6-/- mice but not in sm-cGKI-/- mice. In untreated aorta, α-adrenergic stimulation induced larger contractions in aorta from sm-cGKI-/-, Trpc6-/-, Trpc3-/-/6-/-, and Trpc1-/-/3-/-/6-/- than in those from control and Trpc3-/- mice indicating a functional link between cGKI and TRPC6 channels. TRPC3 channels were detected by immunocytochemistry in both isolated aortic SM cells (SMC) and aortic endothelial cells (EC), whereas TRPC6 channels were detected only in EC. Phenylephrine-stimulated Ca2+ levels were similar in SMC from Ctr and Trpc6 -/- mice. Carbachol-stimulated Ca2+ levels were reduced in EC from Trpc6 -/- mice. Stimulated Ca2+ levels were lowered by 8-Br-cGMP in Ctr but not in Trpc6 -/- EC.

Conclusion

The results suggest that cGKI and TRPC1,3,6 channels are not functionally coupled in vascular SM. Deletion of TRPC6 channels impaired endothelial cGKI signaling and the vasodilator tone in aorta.

Authors’ Affiliations

(1)
FOR 923, Institut für Pharmakologie und Toxikologie, Technische Universität München
(2)
Pharmakologisches Institut, Ruprecht-Karls-Universität
(3)
Experimentelle und Klinische Pharmakologie und Toxikologie, Universität des Saarlandes
(4)
Institut für Pharmakologie und Toxikologie, Ludwig-Maximilians-Universität München
(5)
National Institute of Environmental Health Sciences

Copyright

© Loga et al; licensee BioMed Central Ltd. 2013

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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