Figure 4

U46619 increases cell death in cardiomyocytes. A. HL-1 cardiomyocytes were incubated with increasing concentrations of U46619 (0.1-10 μM) and vehicle for 24 h. U46619 increased cell death at 5 and 10 M as measured by trypan blue staining (n = 3; p < 0.05). B. HL-1 cardiomyocytes were incubated with increasing concentrations of U46619 (1-10 μM) and vehicle for 24 h and viability was also determined by the MTT assay. A decrease in MTT staining was noted at 5 and 10 μM U46619 which indicates a decrease in metabolism and cell viability. Data were presented in terms of increased cell death from vehicle (n = 4, p < 0.05). C. Primary AVCMs were incubated with increasing concentrations of U46619 (1-10 μM) and vehicle for 24 h (image shows AVCMs treated with 10 μM U46619). Culture plates were washed and remaining cells counted (n = 3). Rod-shaped morphology, with clear striations were the criteria used for identifying viable cells, whereas round-shaped myocytes, loss of striation or membrane blebbing were considered non-viable cells. D. A reduction in the number of healthy rod shaped cardiomyocytes was seen at 5 and 10 μM U46619 (n = 3; p < 0.05). *Statistical difference from vehicle.