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NMR study of a soluble Guanylate Cyclase (sGC) human homologue: the H-NOX domain from Nostoc sp.

Heme-nitric oxide/oxygen binding (H-NOX) motifs can be found as proteins of approximately 200 amino acids in length or can exist as a domain within larger proteins, such as soluble guanylate cyclase. The H-NOX domain is conserved across eukaryotes and bacteria; within sGC, the H-NOX domain functions as a sensor for the gaseous signaling agent nitric oxide (NO). Soluble guanylate cyclase (sGC) contains a heme-binding N-terminal domain that regulates the catalytic site contained within the C-terminal end of the enzyme. sGC is a heterodimer, consisting of α1 or α2 subunit bound to β1 and catalyzes the conversion of GTP to GMP. Activation of NO by sGC increases its activity several hundred-fold, promoting vasodilation and inhibiting platelet aggregation. Under pathophysiological conditions characterized by oxidative stress, sGC suffers heme loss, becomes unresponsive to NO and is tagged for degradation by the ubiquitin-proteasome pathway, leading to compromised NO signaling and cardiovascular disease. Ligands, such as BAY 58-2667, activate sGC in a heme-independent manner and protect heme-oxidized sGC from proteasome degradation. Herein, we present a preliminary NMR investigation of the conformational and electronic properties of the heme-bound H-NOX protein from Nostoc sp., which shares a 35% sequence identity with the H-NOX domain of human sGC. Additionally, we use UV-visible and heteronuclear NMR spectroscopy in order to investigate the structural integrity, the conformational variations and the dynamics of the H-NOX polypeptide during oxidation of the Fe(II) ion, while data on the changes/destabilization of the heme moiety upon the addition of a number of ligands and oxidizing agents (NO, BAY 58-2667, ODQ) are acquired through NMR. Monitoring the dynamical behavior of the H-NOX domain and the alterations occurring in its structure triggered by the changes in the oxidation status of the Fe(II)-Protporphyrin IX prosthetic group in solution by NMR, may provide valuable insights for sGC activation/stimulation and NO signaling.

Acknowledgments

We acknowledge partial support from EU FP7-REGPOT-2011 “SEE-DRUG” (nr. 285950 to A.P. & G.S.).

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Correspondence to Andreas Papapetropoulos.

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Argyriou, A., Bantzi, M., Giannis, A. et al. NMR study of a soluble Guanylate Cyclase (sGC) human homologue: the H-NOX domain from Nostoc sp.. BMC Pharmacol Toxicol 16, A73 (2015). https://doi.org/10.1186/2050-6511-16-S1-A73

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Keywords

  • Nitric Oxide
  • Proteasome Degradation
  • Human Homologue
  • Prosthetic Group
  • Inhibit Platelet Aggregation