Fig. 2

Analyses of the effect of DHMEQ treatment on NF-κB activity in CsA nephropathy. a DNA-binding activity of NF-κB (p65) in nuclear and cytoplasmic extracts, as determined by nonradioactive NF-κB-specific DNA-binding ELISA. The results are shown as the relative ratio of DNA-binding activity of NF-κB (p65) in the nucleus to that in the cytoplasm. b Representative immunohistochemical staining of p65 in the control. c Representative immunohistochemical staining of p65 in the CsA group. d Representative immunohistochemical staining of p65 in the CsA + DHMEQ group. All photos are magnified 100×. Arrows indicate nuclei positive for p65 staining. e The graph indicates the number of nuclei positively stained for p65 in each group. The circular, rectangular, and triangular dots represent the data in the control, CsA, and CsA + DHMEQ groups, respectively. The bars represent the mean values ± s.e.m.s.