Fig. 2

Effect of EUG (a), AST (b), DOX plus EUG (c) and DOX plus AST (d) on survival of MCF7 cells. Normalized isobologram constructed for the combination of increasing concentrations of DOX with decreasing concentrations of EUG (e) and AST (f). The combination index produced from 1 mM EUG combined with 0.25 μM DOX (g) and 40 μM AST combined with 0.25 μM DOX (h). Data are expressed as mean ± SD of three separate experiments, each performed in triplicates. ^a indicates significant change from control at P ≤ 0.05 using one-way ANOVA followed by Dunette as a post ANOVA test and ^b indicates significant from DOX alone at P ≤ 0.05 using student t-test.