This article has Open Peer Review reports available.
Distinct single cell signal transduction signatures in leukocyte subsets stimulated with khat extract, amphetamine-like cathinone, cathine or norephedrine
- Therese Bredholt†1,
- Elisabeth Ersvær†1, 2,
- Bjarte Skoe Erikstein1,
- André Sulen1,
- Håkon Reikvam1, 3,
- Hans Jørgen Aarstad4, 5,
- Anne Christine Johannessen5, 6,
- Olav Karsten Vintermyr5, 6,
- Øystein Bruserud1, 3 and
- Bjørn Tore Gjertsen1, 3Email author
© Bredholt et al.; licensee BioMed Central Ltd. 2013
Received: 1 March 2013
Accepted: 3 July 2013
Published: 11 July 2013
Amphetamine and amphetamine derivatives are suggested to induce an immunosuppressive effect. However, knowledge of how amphetamines modulate intracellular signaling pathways in cells of the immune system is limited. We have studied phosphorylation of signal transduction proteins (Akt, CREB, ERK1/2, NF-κB, c-Cbl, STAT1/3/5/6) and stress sensors (p38 MAPK, p53) in human leukocyte subsets following in vitro treatment with the natural amphetamine cathinone, the cathinone derivatives cathine and norephedrine, in comparison with a defined extract of the psychostimulating herb khat (Catha edulis Forsk.). Intracellular protein modifications in single cells were studied using immunostaining and flow cytometry, cell viability was determined by Annexin V-FITC/Propidium Iodide staining, and T-lymphocyte proliferation was measured by 3H-thymidine incorporation.
Cathinone, cathine and norephedrine generally reduced post-translational modifications of intracellular signal transducers in T-lymphocytes, B-lymphocytes, natural killer cells and monocytes, most prominently affecting c-Cbl (pTyr700), ERK1/2 (p-Thr202/p-Tyr204), p38 MAPK (p-Thr180/p-Tyr182) and p53 (both total p53 protein and p-Ser15). In contrast, the botanical khat-extract induced protein phosphorylation of STAT1 (p-Tyr701), STAT6 (p-Tyr641), c-Cbl (pTyr700), ERK1/2 (p-Thr202/p-Tyr204), NF-κB (p-Ser529), Akt (p-Ser473), p38 MAPK (p-Thr180/p-Tyr182), p53 (Ser15) as well as total p53 protein. Cathinone, cathine and norephedrine resulted in unique signaling profiles, with B-lymphocytes and natural killer cells more responsive compared to T-lymphocytes and monocytes. Treatment with norephedrine resulted in significantly increased T-lymphocyte proliferation, whereas khat-extract reduced proliferation and induced cell death.
Single-cell signal transduction analyses of leukocytes distinctively discriminated between stimulation with cathinone and the structurally similar derivatives cathine and norephedrine. Cathinone, cathine and norephedrine reduced phosphorylation of c-Cbl, ERK1/2, p38 MAPK and p53(Ser15), and norephedrine induced T-lymphocyte proliferation. Khat-extract induced protein phosphorylation of signal transducers, p38 MAPK and p53, followed by reduced cell proliferation and cell death. This study suggests that protein modification-specific single-cell analysis of immune cells could unravel pharmacologic effects of amphetamines and amphetamine-like agents, and further could represent a valuable tool in elucidation of mechanism(s) of action of complex botanical extracts.
The herbal drug khat (Catha edulis Forsk.) is chewed for its psychostimulating effects by millions of people [1, 2]. The alkaloid cathinone represents its main psychoactive compound, with a structure and pharmacological profile similar to the synthetic drug amphetamine . Cathinone represents a labile precursor for cathine and norephedrine , being metabolized primarily to norephedrine in vivo. In addition to cathinone and its derivates, khat contains other bioactive constituents, including tannins, ascorbic acid, the phenylalkylamine meruchatine , catheduline alkaloids , flavonoids and triterpenoids [3, 7].
During khat chewing, cathinone, cathine and norephedrine are extracted into saliva, and the oral buccal mucosa plays a major role in absorption . Like amphetamine, cathinone induces release of dopamine, serotonin (5-hydroxytryptamine, 5-HT) and noradrenaline from pre-synaptic neuronal terminals, and has further been suggested to inhibit neurotransmitter reuptake . Cathinone, cathine and norephedrine have been shown to act as substrates at the noradrenaline and dopamine transporters, to bind α2-adrenergic receptors [9, 10], and cathinone has in addition been reported to bind 5-HT receptors [10, 11]. The β1-adrenergic receptor was suggested to mediate khat-induced increase in systolic blood pressure and pulse rate , whereas the α1-adrenergic receptor has been implicated in bladder dysfunction following khat chewing .
Leukocytes express transporters and receptors for dopamine and serotonin, and several types of immune cells store these neurotransmitters (reviewed in [14, 15]). It has further been suggested that lymphocytes are capable of producing dopamine [16, 17]. Leukocytes are known to express adrenergic receptors, and expression of the noradrenaline transporter has also been reported . Various drugs that influence neurotransmitter transport, including anti-depressants and amphetamines, have demonstrated immunosuppressive effects in vivo (reviewed in [14, 19]). Several studies have suggested that at least part of the immunosuppression could be due to direct impact of such pharmacological substrates on immune cells. For instance, House et al. reported that amphetamine could suppress B-lymphocyte proliferation and IL-2 secretion from T-lymphocytes in vitro. Amphetamine was further seen to reduce zymosan-induced phagocytosis both in vitro and in vivo.
Intracellular signal transduction proteins and cell stress sensors have been linked to activation of adrenergic, dopaminergic and serotonergic receptors, which represent G-protein coupled receptors. The β-adrenergic/G stimulatory/protein kinase A pathway was reported to induce phosphorylation of mitogen-activated protein kinase (MAPK) p38 in activated B-lymphocytes  and in T-lineage cells . 5-HT7 receptor stimulation on naïve T-lymphocytes has been suggested to induce activation of extra-cellular regulated kinase 1/2 (ERK1/2) . Further, stimulation of dopaminergic D2-like receptors was reported to reduce phosphorylation of cyclic AMP response element binding (CREB) protein in T-lymphocytes .
In this study we exposed peripheral blood leukocytes in vitro to cathinone, cathine, norephedrine, and to physiological relevant concentrations of khat-extract, and investigated the impact on selected signaling and stress sensor proteins. The distinct signatures of protein modifications detected in the leukocyte subsets indicate cellular effects and mechanism(s) of action of the khat-derived alkaloids, and further demonstrate that the cellular signaling response diverge from treatment with a complex botanical khat-extract.
Cathinone, cathine and norephedrine concentrations in the khat-extract stock solution, and in experimental cell cultures
khat-extract stock solution
1.7 × 10-2
2.0 × 10-2
2.0 × 10-3
2.5 × 10-3
1.7 × 10-5
3 × 10-3
2.0 × 10-5
3 × 10-4
2.0 × 10-6
3.16 × 10-4 dilution
7.9 × 10-4
5.4 × 10-6
9.5 × 10-4
6.3 × 10-6
9.5 × 10-5
6.3 × 10-7
Signal transduction in T-lymphocytes
For T-lymphocytes (Figure 1B), treatment with the lowest khat-extract concentration induced p-STAT1, p-p38 MAPK, p-STAT6 and p-AKT; the highest khat-extract concentration induced p-c-Cbl, p-ERK1/2, p-p38 MAPK, p53 p-Serine (Ser)15 and total p53; cathine reduced p-c-Cbl, p-ERK1/2, p-p38 MAPK, p53 p-Ser15 and total p53; the combination norephedrine/cathinone reduced p-c-Cbl and p53 p-Ser15. The time-points and p-values for these changes are listed in Additional file 1: Table S1.
The cluster analysis of signal transduction in T-lymphocytes following 15 minutes of stimulation present significant and non-significant changes (Figure 1C). Khat-extract generally increased protein phosphorylation, in contrast to cathinone, cathine and norephedrine, which reduced phosphorylation of several proteins. Cathinone and its derivatives clustered separately from khat-extract, indicating different pharmacological properties of the complex extract compared to the pure khat components.
Signal transduction in B-lymphocytes
For B-lymphocytes (Figure 2A), treatment with the lowest khat-extract concentration induced p-STAT1, p-p38 MAPK, p-STAT6, p-AKT and p53 p-Ser37; the highest concentration of khat-extract induced p-STAT1, p-c-Cbl, p-ERK1/2, p-STAT3, p-NF-κB, p-p38 MAPK, p53 p-Ser15 and total p53. In contrast, cathine reduced p-c-Cbl, p-ERK1/2, p-p38 MAPK, p53 p-Ser15 and total p53; the combination norephedrine/cathinone reduced p53 p-Ser15. The time-points and p-values for these changes are listed in Additional file 1: Table S1.
The cluster analysis of signal transduction in B-lymphocytes (15 minutes) displays significant and non-significant changes (Figure 2B). As in T-lymphocytes, khat-extract induced protein phosphorylation, whereas cathinone and its derivatives decreased phosphorylation levels. In particular, p-c-Cbl, p-ERK1/2, p-p38- MAPK and p53 p-Ser15 were prominently reduced, and these phosphorylation responses clustered together.
Signal transduction in natural killer cells
For NK cells (Figure 3A), treatment with the lowest khat-extract concentration induced p-STAT1, p-STAT3, p-NF-κB, p-STAT6, p-AKT and total p53; the highest khat-extract concentration induced p-c-Cbl, p-ERK1/2, p-STAT3, p-NF-κB, p-p38 MAPK, p53 p-Ser15 and total p53; norephedrine induced p-STAT3; cathine reduced p-c-Cbl, p-ERK1/2, p-p38 MAPK and p53 p-Ser15. The time-points and p-values for these changes are listed in Additional file 1: Table S1. The cluster analysis of signal transduction in NK cells (15 minutes) displays significant and non-significant changes (Figure 3B). In NK cells, cathinone and its derivatives both induced and decreased protein phosphorylation.
Signal transduction in monocytes
For monocytes (Figure 4A), treatment with the lowest khat-extract concentration induced p-STAT1, p-p38 MAPK, p-STAT6 and p-AKT; the highest khat-extract concentration induced p-c-Cbl, p-ERK1/2, p-p38 MAPK, p53 p-Ser15 and total p53; cathine reduced p-c-Cbl and p53 p-Ser15. The cluster analysis of signal transduction in monocytes (15 minutes) indicates significant and non-significant changes (Figure 4B). Cathinone and its derivatives gave a less pronounced reduction in phosphorylation levels in the monocyte subset.
Alterations in p53 post-translational modifications
For T-lymphocytes, the highest khat-extract concentration reduced p-Ser37; cathine, cathinone and the combination norephedrine/cathinone reduced p-Ser15. For B-lymphocytes, cathine, cathinone and norephedrine/cathinone reduced p-Ser15, while cathine increased p-Ser37. For NK cells, the highest khat-extract concentration reduced p-Ser37 and ac-Lys382; cathine, cathinone and norephedrine/cathinone all reduced p-Ser15. In monocytes, the highest khat-extract concentration reduced ac-Lys382; cathine reduced p-Ser15. The observations of modifications of central signal transduction pathways and stress proteins were extended with analyses of effects on cell death and proliferation in an attempt to connect early signal transduction responses with later functional effects on leukocytes.
Khat, cathinone and its derivatives affected leukocyte viability and proliferation
Effects on T-lymphocyte proliferation were assessed following 4 days of stimulation, based on 3H-thymidine incorporation (Figure 6B). The highest khat-extract concentration inhibited proliferation by approximately 50%. Treatment with cathinone and cathine gave elevated CPMA values, and norephedrine significantly increased T-lymphocyte proliferation (p < 0.008).
Cathinone and its natural derivatives generally suppressed basal phosphorylation of the examined signal transducers and stress sensors, while khat-extract induced protein post-translational modifications (Figures 1, 2, 3 and 4). The different impact on signal transduction proteins in the leukocytes was reflected in the functional assays, demonstrating khat-induced cell death and reduced T-lymphocyte proliferation, whereas no cytotoxic effects were detected for cathinone, cathine and norephedrine (Figure 6). Toxic effects by khat have been reported in in vivo and in vitro studies [3, 30–32]. We have previously shown that khat induce caspase-dependent apoptosis in myeloid cells and keratinocytes [30, 31, 33]. Khat-induced apoptosis may involve induction of reactive oxygen species  (see also Aleruyani et al. for review ), which is described to function upstream of p53 and p38 in some model systems, while in others ROS production could be a downstream effect of p53 activation [35, 36]. We therefore anticipated that single cell signalling analysis of total and modified p53 and phosphorylated p38 MAPK should reveal the mechanisms involved in khat toxicity when compared with cathinone and its natural derivatives.
There are conflicting results regarding toxic effects by cathinone and its natural derivatives [20, 26, 33]. Here we observed that norephedrine significantly increased T-lymphocyte proliferation after long-time exposure. A study by House and co-workers, comparing synthetic amphetamines and cathinone, demonstrated that cathinone induced B-lymphocyte proliferation, cytotoxic T-lymphocyte induction and IL-2 production . As far as we know, a cell proliferative effect by norephedrine has not previously been demonstrated in vitro.
We have previously reported that khat-extract induced the tumor suppressor and cellular stress sensor p53 in normal oral keratinocytes and fibroblasts . Here we demonstrate that p53 protein accumulates in leukocytes following khat-extract treatment, accompanied by, in particular, increased Ser15 phosphorylation (Figures 1, 2, 3 and 4). In contrast to khat-extract, cathinone and its derivatives generally reduced total p53 levels, p53 Ser15 phosphorylation (Figures 1, 2, 3 and 4) and Ser15 phosphorylation was further seen reduced relative to total p53 (Figure 5).
Cathine significantly reduced p38 MAPK phosphorylation in the various leukocyte subsets, whereas khat-extract mediated a significant increase in phosphorylation of this cell stress protein. In agreement with our observations, khat was recently reported to induce p38 MAPK phosphorylation in PBMCs in vitro, and khat-treatment was seen to induce cell death by apoptosis . p38 is probably acting upstream of p53, and may be pivotal in khat-induced abnormal differentiation of in vitro-reconstructed human normal buccal mucosa . Additionally, our data indicate that in particular cathine attenuated c-Cbl, ERK1/2 and p53 Ser15 phosphorylation in the various subsets, further including p-STAT6 and p-Akt reduction in T-lymphocytes (Figure 1). Interestingly, c-Cbl was recently reported to play an important role in trafficking of the 5-HT2A receptor in human embryonic kidney cells , whereas de-phosphorylation of AKT was observed following stimulation of striatal class D2 dopamine receptors [39, 40].
Our results suggest that cathinone and its derivatives generally reduced basal phosphorylation levels of the proteins analysed, but as an exception, norephedrine was seen to significantly induce phosphorylation of STAT3 in NK cells. STAT3 is known to induce both proliferative and anti-proliferative effects, but a recent study suggested that STAT3 could mediate an immunosuppressive effect in NK cells . Further, even if not statistical significant, cathine and cathinone indicated elevated p-CREB levels in all leukocyte subsets, whereas norephedrine reduced basal phosphorylation of CREB. Our results may indicate that the alkaloids cathine and cathinone induce CREB phosphorylation as has been shown for e.g. ephedrine. Ephedrine, an alkaloid chemically similar to cathinone, has been suggested to induce PKA-mediated CREB phosphorylation through β-adrenergic receptor/cyclic AMP/PKA/DARPP-32 signaling pathway . Taken together these observations show that cathinone, cathine and norephedrine resulted in distinct signaling signatures, even if the compounds are closely related structurally.
The leukocyte subsets differed in their signaling responses to the various treatments, and B-lymphocytes and NK cells were generally seen as more responsive compared to T-lymphocytes and monocytes. This observation could relate to varying levels or different repertoires of monoamine transporters and receptors on the immune cells. For instance, T-lymphocytes and monocytes were reported to exhibit a low expression of dopamine receptors when compared to B-lymphocytes and NK cells, which displayed a higher and more consistent expression . The mechanism(s) behind the different responses to khat-extract, cathinone and its derivatives seen in the various leukocyte subsets should be addressed in future studies.
In recent years, synthetic cathinone derivatives have been designed, being widely distributed as recreational drugs. The use of these drugs has been reported with harmful health effects, and has been suspected to be involved in several deaths . Signal transduction studies and functional assays of synthetic cathinone derivatives and other amphetamine-like agents, like the ones performed here, could be useful in evaluation of potential harmful short term and long term effects on various immune cells. In this study we observed significant changes in phosphorylation levels for several of the investigated signal transduction and cell stress proteins, but the changes were still modest. In order to evaluate more fully the impact of pharmacological substrates on cells of the immune system, the approach should be extended and involve analyses of immune cells following in vivo studies with experimental animals.
We report that the khat-derived cathinone and its derivatives generally reduced the levels of activating protein modifications of signal transducers and stress sensors in leukocytes, and that norephedrine increased in vitro T-lymphocyte proliferation. The closely structurally related compounds cathinone, cathine and norephedrine were seen to induce unique signaling signatures in leukocytes. Further, this study confirmed that compounds in a botanical khat-extract activated cell stress sensors in immune cells and induced cytotoxic effects. Our results suggest that single-cell flow cytometric analysis of intracellular signaling pathways may be an informative and feasible approach when elucidating pharmacological effects and mechanisms of action in immune cells.
Blood was obtained from healthy donors from Department of Immunology and Transfusion Medicine, Haukeland University Hospital, Bergen, Norway. The blood donors used in the signal transduction analyses were 6 healthy individuals (4 females, 2 males) with a mean age of 38.2 years (range: 24–59). The use of blood for research purposes was approved by the local ethics committee REK (Regionale komiteer for medisinsk og helsefaglig forskningsettikk), Haukeland University Hospital, and samples collected after written consent by the blood donors, in accordance with the Declaration of Helsinki.
Cathinone, cathine and norephedrine
S-(−)-cathinone, (1S,2S)-(+)-norpseudoephedrine (cathine) and (1R,2S)-(−)-norephedrine were purchased from Sigma-Aldrich, and dissolved in DMSO (Sigma-Aldrich). These compounds were used in cell cultures at the concentration 10-4 M, and import and experiments were performed after written permission from The Norwegian Medicines Agency.
Khat (asili cultivate) from the Meru district in Kenya was purchased in Nairobi, transported at 4°C under moist conditions, and the material was extracted within 48 hrs of harvest with methanol [30, 33]. The khat-extract was prepared from approximately 200 g of khat, using the leaves and the soft parts of the twigs, which were chopped into pieces of approximately 5 mm length and submerged in methanol. The methanol extract was dried using a rotary evaporator, the semi-solid residue dissolved at a concentration of 0.2 g/ml in dimethylsulphoxide (DMSO; Sigma, St. Louis, MO, USA) and the stock solution stored at −80°C.
The stock solution was diluted in Roswell Park Memorial Institute (RPMI)-1640 medium (Sigma-Aldrich) with 10% fetal bovine serum (FBS), 2 mM L-glutamine and antibiotics (100 IU ml-1 penicillin and 100 μg ml-1 streptomycin; Gibco, Grand Island, NY, USA), and precipitates removed by centrifugation (10 000 g, 15 minutes, 4°C) . The khat-extract supernatant was added experimental cell cultures giving final dilutions of 10-3 and 3.16 × 10-4, based on previous studies . All experimental cell cultures contained 0.1% DMSO.
Cathinone is relatively unstable, being transformed to cathine upon wilting of khat leaves, while being metabolized predominantly to norephedrine in vivo[3, 4]. Cathinone, cathine and norephedrine are therefore suited as reference substances, indicating the freshness of the khat sample and the stability of the khat-extract. The concentrations of cathinone, cathine and norephedrine in the khat-extract were determined using high pressure liquid chromatography and mass spectrometry (HPLC-MS-MS), as previously described , and the values are displayed in Table 1, together with experimental cell culture concentrations.
In order to import and study khat, classified as an illegal narcotic substance in Norway, written permission was obtained from The Norwegian Medicines Agency (permission 01/705 of 2002-07-08).
Peripheral blood mononuclear cells
Peripheral blood mononuclear cells (PBMCs) were isolated from buffy coats using density gradient separation (Lymphoprep; Nycomed, Oslo, Norway; specific density 1.077), and resuspended at: (i) 1 × 106 cells ml-1 in X-vivo10® culture medium (BioWhittaker, Walkersville, MA, USA) with 100 μg ml-1 gentamicin for use in proliferation studies, (ii) 1 × 106 cells ml-1 in RPMI-1640 medium (10% FBS, L-glutamine, antibiotics) for studies on signal transduction, or (iii) 200 000 cells ml-1 in RPMI-1640 medium (10% FBS, L-glutamine, antibiotics) when determining apoptosis.
Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) staining
When determining apoptosis, the PBMCs were stained with Annexin V-FITC and PI, using the Apoptest™ kit from Nexins Research (Hoeven, the Netherlands) according to the manufacturer’s protocol. Data were collected on a FACS Calibur flow cytometer (Becton Dickinson, BD, Immunocytometry Systems, San Jose, CA, USA) and analyzed with FlowJo 7.2.5 software (TreeStar Inc., Ashland, OR, USA).
3H-thymidine proliferation assay
The assay used to determine in vitro lymphocyte proliferation is based on a whole blood protocol . Experiments were performed in triplicates in 96-well plates, and each well added (i) 5 × 104 PBMCs; (ii) CD3 antibody (mouse IgE Moab; CLB-T3/4.E), final concentration 316 ng ml-1; (iii) CD28 antibody (mouse IgG1 Moab, CLB-CD28/1), final concentration 842 ng ml-1; and (iv) test compound (cathinone, cathine, norephedrine, khat-extract) or control medium. The cell cultures were incubated (37°C, 5% CO2) for 3 days, added 3H-thymidine (37 kBq per well; TRA 310, Amersham International, Amersham, UK), and nuclear radioactivity assayed on day four by liquid scintillation counting. Results are expressed as percent average counts per minute (CPMA) in treated cells relative to CPMA in controls: (CPMAtreated/CPMAcontrol) × 100. All CPMA values were >1000, indicating proliferating cells.
Intracellular staining of proteins and flow cytometric analyses
Antibodies used in this study
Alexa Fluor® 647
Alexa Fluor® 647
Alexa Fluor® 488
Alexa Fluor® 488
Alexa Fluor® 647
Alexa Fluor® 488
Alexa Fluor® 647
Alexa Fluor® 647
Alexa Fluor® 647
Alexa Fluor® 488
Cell Signaling Technologies
Alexa Fluor® 647
Alexa Fluor® 488
Alexa Fluor® 488
Statistical and bioinformatical approaches
Heatmaps: Statistical comparisons were made using GraphPad PRISM (version 5.0, GraphPad Software, Inc., USA) software, with one-way analysis of variance (ANOVA) and Tukey’s multiple comparison post-tests to determine significant differences. A Student’s unpaired or paired t-test was employed when only two groups were compared. Cluster analyses: Analysis were performed using J-Express 2011 analysis suite (MolMine AS, Bergen, Norway) . For each protein the mean fold change for the donors was calculated, for each leukocyte subset and each time point. Values were log(2) converted before unsupervised hierarchical clustering analyses were performed using Euclidean correlation with complete linkage.
This work was supported by funding from the following grants: The Norwegian Cancer Society with Solveig and Ole Lunds Legacy, the Western Regional Norwegian Authority (Helse-Vest), and the Norwegian Research Council with Programme in Functional Genomics. The authors would like to thank Kåre Strønen for his valuable contribution to improve the figures.
- Al-Hebshi NN, Skaug N: Khat (Catha edulis)-an updated review. Addict Biol. 2005, 10 (4): 299-307. 10.1080/13556210500353020.View ArticlePubMedGoogle Scholar
- Al-Motarreb A, Baker K, Broadley KJ: Khat: pharmacological and medical aspects and its social use in Yemen. Phytother Res. 2002, 16 (5): 403-413. 10.1002/ptr.1106.View ArticlePubMedGoogle Scholar
- Kalix P: Pharmacological properties of the stimulant khat. Pharmacol Ther. 1990, 48 (3): 397-416. 10.1016/0163-7258(90)90057-9.View ArticlePubMedGoogle Scholar
- Toennes SW, Kauert GF: Excretion and detection of cathinone, cathine, and phenylpropanolamine in urine after kath chewing. Clin Chem. 2002, 48 (10): 1715-1719.PubMedGoogle Scholar
- Brenneisen R, Geisshulser S, Schorno X: Merucathine, A New Phenylalkylamine from Catha edulis. Planta Med. 1984, 50 (6): 531-10.1055/s-2007-969796.View ArticlePubMedGoogle Scholar
- Crombie L: The cathedulin alkaloids. Bull Narc. 1980, 32 (3): 37-50.PubMedGoogle Scholar
- Halbach H: Medical aspects of the chewing of khat leaves. Bull World Health Organ. 1972, 47 (1): 21-29.PubMedPubMed CentralGoogle Scholar
- Toennes SW, Harder S, Schramm M, Niess C, Kauert GF: Pharmacokinetics of cathinone, cathine and norephedrine after the chewing of khat leaves. Br J Clin Pharmacol. 2003, 56 (1): 125-130. 10.1046/j.1365-2125.2003.01834.x.View ArticlePubMedPubMed CentralGoogle Scholar
- Cleary L, Docherty JR: Actions of amphetamine derivatives and cathinone at the noradrenaline transporter. Eur J Pharmacol. 2003, 476 (1–2): 31-34.View ArticlePubMedGoogle Scholar
- Rothman RB, Vu N, Partilla JS, Roth BL, Hufeisen SJ, Compton-Toth BA, Birkes J, Young R, Glennon RA: In vitro characterization of ephedrine-related stereoisomers at biogenic amine transporters and the receptorome reveals selective actions as norepinephrine transporter substrates. J Pharmacol Exp Ther. 2003, 307 (1): 138-145. 10.1124/jpet.103.053975.View ArticlePubMedGoogle Scholar
- Glennon RA, Liebowitz SM: Serotonin receptor affinity of cathinone and related analogues. J Med Chem. 1982, 25 (4): 393-397. 10.1021/jm00346a012.View ArticlePubMedGoogle Scholar
- Hassan NA, Gunaid AA, El-Khally FM, Al-Noami MY, Murray-Lyon IM: Khat chewing and arterial blood pressure. A randomized controlled clinical trial of alpha-1 and selective beta-1 adrenoceptor blockade. Saudi Med J. 2005, 26 (4): 537-541.PubMedGoogle Scholar
- Nasher AA, Qirbi AA, Ghafoor MA, Catterall A, Thompson A, Ramsay JW, Murray-Lyon IM: Khat chewing and bladder neck dysfunction. A randomized controlled trial of alpha 1-adrenergic blockade. Br J Urol. 1995, 75 (5): 597-598. 10.1111/j.1464-410X.1995.tb07415.x.View ArticlePubMedGoogle Scholar
- Gordon J, Barnes NM: Lymphocytes transport serotonin and dopamine: agony or ecstasy?. Trends Immunol. 2003, 24 (8): 438-443. 10.1016/S1471-4906(03)00176-5.View ArticlePubMedGoogle Scholar
- Meredith EJ, Chamba A, Holder MJ, Barnes NM, Gordon J: Close encounters of the monoamine kind: immune cells betray their nervous disposition. Immunology. 2005, 115 (3): 289-295. 10.1111/j.1365-2567.2005.02166.x.View ArticlePubMedPubMed CentralGoogle Scholar
- Josefsson E, Bergquist J, Ekman R, Tarkowski A: Catecholamines are synthesized by mouse lymphocytes and regulate function of these cells by induction of apoptosis. Immunology. 1996, 88 (1): 140-146. 10.1046/j.1365-2567.1996.d01-653.x.View ArticlePubMedPubMed CentralGoogle Scholar
- Bergquist J, Silberring J: Identification of catecholamines in the immune system by electrospray ionization mass spectrometry. Rapid Commun Mass Spectrom. 1998, 12 (11): 683-688. 10.1002/(SICI)1097-0231(19980615)12:11<683::AID-RCM218>3.0.CO;2-N.View ArticlePubMedGoogle Scholar
- Mata S, Urbina M, Manzano E, Ortiz T, Lima L: Noradrenaline transporter and its turnover rate are decreased in blood lymphocytes of patients with major depression. J Neuroimmunol. 2005, 170 (1–2): 134-140.View ArticlePubMedGoogle Scholar
- Boyle NT, Connor TJ: Methylenedioxymethamphetamine (‘Ecstasy’)-induced immunosuppression: a cause for concern?. Br J Pharmacol. 2010, 161 (1): 17-32. 10.1111/j.1476-5381.2010.00899.x.View ArticlePubMedPubMed CentralGoogle Scholar
- House RV, Thomas PT, Bhargava HN: Comparison of immune functional parameters following in vitro exposure to natural and synthetic amphetamines. Immunopharmacol Immunotoxicol. 1994, 16 (1): 1-21. 10.3109/08923979409029897.View ArticlePubMedGoogle Scholar
- Freire-Garabal M, Nunez MJ, Balboa JL, Fernandez-Rial JC, Belmonte A: Effects of amphetamine on the activity of phagocytosis in mice. Life Sci. 1992, 51 (15): PL145-PL148. 10.1016/0024-3205(92)90362-S.View ArticlePubMedGoogle Scholar
- McAlees JW, Sanders VM: Hematopoietic protein tyrosine phosphatase mediates beta2-adrenergic receptor-induced regulation of p38 mitogen-activated protein kinase in B lymphocytes. Mol Cell Biol. 2009, 29 (3): 675-686. 10.1128/MCB.01466-08.View ArticlePubMedGoogle Scholar
- Lajevic MD, Suleiman S, Cohen RL, Chambers DA: Activation of p38 mitogen-activated protein kinase by norepinephrine in T-lineage cells. Immunology. 2011, 132 (2): 197-208. 10.1111/j.1365-2567.2010.03354.x.View ArticlePubMedPubMed CentralGoogle Scholar
- Leon-Ponte M, Ahern GP, O’Connell PJ: Serotonin provides an accessory signal to enhance T-cell activation by signaling through the 5-HT7 receptor. Blood. 2007, 109 (8): 3139-3146. 10.1182/blood-2006-10-052787.View ArticlePubMedPubMed CentralGoogle Scholar
- Huang Y, Qiu AW, Peng YP, Liu Y, Huang HW, Qiu YH: Roles of dopamine receptor subtypes in mediating modulation of T lymphocyte function. Neuro Endocrinol Lett. 2010, 31 (6): 782-791.PubMedGoogle Scholar
- Lukandu OM, Costea DE, Dimba EA, Neppelberg E, Bredholt T, Gjertsen BT, Vintermyr OK, Johannessen AC: Khat induces G1-phase arrest and increased expression of stress-sensitive p53 and p16 proteins in normal human oral keratinocytes and fibroblasts. Eur J Oral Sci. 2008, 116 (1): 23-30. 10.1111/j.1600-0722.2007.00508.x.View ArticlePubMedGoogle Scholar
- Sachs K, Perez O, Pe’er D, Lauffenburger DA, Nolan GP: Causal protein-signaling networks derived from multiparameter single-cell data. Science. 2005, 308 (5721): 523-529. 10.1126/science.1105809.View ArticlePubMedGoogle Scholar
- Krutzik PO, Crane JM, Clutter MR, Nolan GP: High-content single-cell drug screening with phosphospecific flow cytometry. Nat Chem Biol. 2008, 4 (2): 132-142. 10.1038/nchembio.2007.59.View ArticlePubMedGoogle Scholar
- Krutzik PO, Nolan GP: Fluorescent cell barcoding in flow cytometry allows high-throughput drug screening and signaling profiling. Nat Methods. 2006, 3 (5): 361-368. 10.1038/nmeth872.View ArticlePubMedGoogle Scholar
- Bredholt T, Dimba EA, Hagland HR, Wergeland L, Skavland J, Fossan KO, Tronstad KJ, Johannessen AC, Vintermyr OK, Gjertsen BT: Camptothecin and khat (Catha edulis Forsk.) induced distinct cell death phenotypes involving modulation of c-FLIPL, Mcl-1, procaspase-8 and mitochondrial function in acute myeloid leukemia cell lines. Mol Cancer. 2009, 8: 101-View ArticlePubMedPubMed CentralGoogle Scholar
- Lukandu OM, Costea DE, Neppelberg E, Johannessen AC, Vintermyr OK: Khat (Catha edulis) induces reactive oxygen species and apoptosis in normal human oral keratinocytes and fibroblasts. Toxicol Sci. 2008, 103 (2): 311-324. 10.1093/toxsci/kfn044.View ArticlePubMedGoogle Scholar
- Murdoch C, Aziz HA, Fang HY, Jezan H, Musaid R, Muthana M: Khat (Catha edulis) alters the phenotype and anti-microbial activity of peripheral blood mononuclear cells. J Ethnopharmacol. 2011, 138 (3): 780-787. 10.1016/j.jep.2011.10.030.View ArticlePubMedGoogle Scholar
- Dimba EA, Gjertsen BT, Bredholt T, Fossan KO, Costea DE, Francis GW, Johannessen AC, Vintermyr OK: Khat (Catha edulis)-induced apoptosis is inhibited by antagonists of caspase-1 and −8 in human leukaemia cells. Br J Cancer. 2004, 91 (9): 1726-1734.PubMedPubMed CentralGoogle Scholar
- Aleryani SL, Aleryani RA, Al-Akwa AA: Khat a drug of abuse: roles of free radicals and antioxidants. Drug Test Anal. 2011, 3 (9): 548-551. 10.1002/dta.224.View ArticlePubMedGoogle Scholar
- Maillet A, Pervaiz S: Redox regulation of p53, redox effectors regulated by p53: a subtle balance. Antioxid Redox Signal. 2012, 16 (11): 1285-1294. 10.1089/ars.2011.4434.View ArticlePubMedGoogle Scholar
- Matsuzawa A, Ichijo H: Redox control of cell fate by MAP kinase: physiological roles of ASK1-MAP kinase pathway in stress signaling. Biochim Biophys Acta. 2008, 1780 (11): 1325-1336. 10.1016/j.bbagen.2007.12.011.View ArticlePubMedGoogle Scholar
- Lukandu OM, Neppelberg E, Vintermyr OK, Johannessen AC, Costea DE: Khat alters the phenotype of in vitro-reconstructed human oral mucosa. J Dent Res. 2010, 89 (3): 270-275. 10.1177/0022034509354980.View ArticlePubMedGoogle Scholar
- Baldys A, Raymond JR: Role of c-Cbl carboxyl terminus in serotonin 5-HT2A receptor recycling and resensitization. J Biol Chem. 2011, 286 (28): 24656-24665. 10.1074/jbc.M110.119891.View ArticlePubMedPubMed CentralGoogle Scholar
- Beaulieu JM, Sotnikova TD, Marion S, Lefkowitz RJ, Gainetdinov RR, Caron MG: An Akt/beta-arrestin 2/PP2A signaling complex mediates dopaminergic neurotransmission and behavior. Cell. 2005, 122 (2): 261-273. 10.1016/j.cell.2005.05.012.View ArticlePubMedGoogle Scholar
- Beaulieu JM, Sotnikova TD, Yao WD, Kockeritz L, Woodgett JR, Gainetdinov RR, Caron MG: Lithium antagonizes dopamine-dependent behaviors mediated by an AKT/glycogen synthase kinase 3 signaling cascade. Proc Natl Acad Sci U S A. 2004, 101 (14): 5099-5104. 10.1073/pnas.0307921101.View ArticlePubMedPubMed CentralGoogle Scholar
- Bedel R, Thiery-Vuillemin A, Grandclement C, Balland J, Remy-Martin JP, Kantelip B, Pallandre JR, Pivot X, Ferrand C, Tiberghien P: Novel role for STAT3 in transcriptional regulation of NK immune cell targeting receptor MICA on cancer cells. Cancer Res. 2011, 71 (5): 1615-1626. 10.1158/0008-5472.CAN-09-4540.View ArticlePubMedGoogle Scholar
- Jia JJ, Zeng XS, Li Y, Ma S, Bai J: Ephedrine induced thioredoxin-1 expression through beta-adrenergic receptor/cyclic AMP/protein kinase A/dopamine- and cyclic AMP-regulated phosphoprotein signaling pathway. Cell Signal. 2013, 25 (5): 1194-1201. 10.1016/j.cellsig.2013.02.007.View ArticlePubMedGoogle Scholar
- McKenna F, McLaughlin PJ, Lewis BJ, Sibbring GC, Cummerson JA, Bowen-Jones D, Moots RJ: Dopamine receptor expression on human T- and B-lymphocytes, monocytes, neutrophils, eosinophils and NK cells: a flow cytometric study. J Neuroimmunol. 2002, 132 (1–2): 34-40.View ArticlePubMedGoogle Scholar
- Morris K: UK places generic ban on mephedrone drug family. Lancet. 2010, 375 (9723): 1333-1334. 10.1016/S0140-6736(10)60559-4.View ArticlePubMedGoogle Scholar
- Wendelbo O, Bruserud O: Functional evaluation of proliferative T cell responses in patients with severe T lymphopenia: characterization of optimal culture conditions and standardized activation signals for a simple whole blood assay. J Hematother Stem Cell Res. 2003, 12 (5): 525-535. 10.1089/152581603322448231.View ArticlePubMedGoogle Scholar
- Irish JM, Anensen N, Hovland R, Skavland J, Borresen-Dale AL, Bruserud O, Nolan GP, Gjertsen BT: Flt3 Y591 duplication and Bcl-2 overexpression are detected in acute myeloid leukemia cells with high levels of phosphorylated wild-type p53. Blood. 2007, 109 (6): 2589-2596. 10.1182/blood-2006-02-004234.View ArticlePubMedGoogle Scholar
- Stavrum AK, Petersen K, Jonassen I, Dysvik B: Analysis of gene-expression data using J-Express. Curr Protoc Bioinformatics. 2008, -Chapter 7:Unit 7 3Google Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/2050-6511/14/35/prepub
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.