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Chronic beta-adrenergic blockade prevents volume overload-induced re-localization and oxidation of soluble guanylyl cyclase
BMC Pharmacology and Toxicology volume 14, Article number: O22 (2013)
Background
While β-adrenergic blockade is a cornerstone of heart failure therapy, its therapeutic role in chronic mitral regurgitation remains questionable. Animal studies and a small clinical trial have demonstrated cardiac functional improvement with β1-adrenoceptor blocker metoprolol in chronic mitral regurgitation [1, 2]. How β1AR-blockade halts functional decline of the volume-overloaded, eccentric hypertrophied heart is not well understood; anti-oxidant effects of β-blockade (βB) may play a role. We recently demonstrated that volume-overload cardiac stress induces re-localization and microdomain-specific oxidation of the nitric oxide receptor soluble guanylyl cyclase (sGC) in the failing heart [3, 4]. Given that nitric oxide-cyclic guanosine monophosphate (NO-cGMP) modulates cardiac contractility and protects against cardiac hypertrophy, we hypothesized that β1AR-blockade prevents oxidation of sGC and promotes myocardial NO-cGMP signaling in a microdomain-specific fashion.
Materials and methods
Volume-overload (VO) was established by chordal rupture-induced mitral regurgitation (MR) in mongrel dogs. Some dogs were treated with metoprolol succinate (100mg orally once daily; MR+ βB). Expression, localization, cyclase activity, and redox state of myocardial sGC were assessed in Control, MR, and MR+βB dogs.
Results
sGCα1 and -β1 subunits were detected within and outside of caveolae-enriched lipid rafts (Cav3+LR). In MR, total sGCα1 expression fell to nearly 50% of Control and re-localized away from Cav3+LR to non-lipid raft microdomains (NLR). While overall sGCβ1 expression was also less in MR+βB, caveolae-localization of sGCβ1 was preserved. Overall NO-responsiveness of sGC remained intact in MR hearts, irrespective of βB therapy. However, a potentiated response to heme/NO-independent sGC activator BAY 60-2770 suggested that a subset of sGC was heme-oxidized in MR but not in Control or MR+βB. Moreover, differential responses to BAY 60-2770 and NO were noted in Cav3+LR and NLR microdomains. In Control hearts, responses to BAY 60-2770 and NO were similar within respective microdomains, suggesting a predominantly reduced form of sGC in both Cav3+LR and NLR of Controls. In contrast, BAY 60-2770 response of NLR-localized sGC was potentiated in MR but not in MR+βB hearts, suggesting that βB therapy prevented oxidation of NLR-localized sGC. Moreover, BAY 60-2770 responses of Cav3+LR-localized sGC were not potentiated in any hearts, suggesting an anti-oxidation protection associated with caveolae-localization. These changes in caveolae-localization and redox state of sGC were also reflected by microdomain distribution of VASP phosphorylation.
Conclusion
β1AR blocker mediated cardioprotection in the volume-overloaded heart is associated with enhanced microdomain specific myocardial NO-cGMP signaling, both within and outside of caveolae. Such prevention of volume overload-induced spatial and redox dysregulation of myocardial sGC suggests novel strategies to enhancing cardioprotective NO-cGMP signaling.
References
- 1.
Pat B, Killingsworth C, Denney T, Zheng J, Powell P, Tillson M, Dillon AR, Dell’Italia LJ: Dissociation between cardiomyocyte function and remodeling in beta-adrenergic receptor blockade in isolated canine mitral regurgitation. Am J Physiol Heart Circ Physiol. 2008, 295: H2321-H2327. 10.1152/ajpheart.00746.2008.
- 2.
Ahmed MI, Aban I, Lloyd SG, Gupta H, Howard G, Inusah S, Peri K, Robinson J, Smith P, McGiffin DC, Schiros CG, Denney T, Dell’Italia LJ: A randomized controlled phase IIb trial of beta(1)-receptor blockade for chronic degenerative mitral regurgitation. J Am Coll Cardiol. 2012, 60: 833-838. 10.1016/j.jacc.2012.04.029.
- 3.
Tsai EJ, Liu Y, Koitabashi N, Bedja D, Danner T, Jasmin JF, Lisanti MP, Friebe A, Takimoto E, Kass DA: Pressure-overload induced subcellular relocalization/oxidation of soluble guanylyl cyclase in the heart modulates enzyme stimulation. Circ Res. 2012, 110: 295-303. 10.1161/CIRCRESAHA.111.259242.
- 4.
Liu Y, Dillon AR, Tillson M, Makarewich C, Nguen V, Dell’Italia L, Sabria A, Rizzo V, Tsai EJ: Volume overload induces differential spatiotemporal regulation of myocardial soluble guanylyl cyclase in eccentric hypertrophy and heart failure. J Mol Cell Cardiol. 2013, 60: 72-83.
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Liu, Y., Dell’Italia, L., Rizzo, V. et al. Chronic beta-adrenergic blockade prevents volume overload-induced re-localization and oxidation of soluble guanylyl cyclase. BMC Pharmacol Toxicol 14, O22 (2013). https://doi.org/10.1186/2050-6511-14-S1-O22
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Keywords
- Metoprolol
- Mitral Regurgitation
- Soluble Guanylyl Cyclase
- VASP Phosphorylation
- Metoprolol Succinate
