- Poster presentation
- Open Access
The soluble guanylyl cyclase activator BAY 60-2770 ameliorates detrusor dysfunction in obese mice
© de Nucci et al; licensee BioMed Central Ltd. 2013
- Published: 29 August 2013
- Contractile Response
- Obese Mouse
- Obese Group
- Bladder Tissue
- cGMP Level
Mice were fed for 12 weeks with either a standard chow diet (carbohydrate: 70%; protein: 20%; fat: 10%) or a high fat diet that induces obesity (carbohydrate: 29%; protein: 16%; fat: 55%). Lean and obese mice were orally treated with BAY 60-2770 (1 mg/kg/day, given as daily gavage from the 10th to the 12th week) or its vehicle (Transcutol®:Cremophor®:water, 1:2:7, v/v/v). Concentration-response curves to full agonist carbachol (CCh, 0.001-100 µM) were obtained. The values of potency (pEC50) and maximal responses (Emax) were calculated. The cGMP levels and Western blotting for α1 and β1-subunit of sGC in the bladder tissues were also determined.
Chronic treatment with BAY 60-2770 results in amelioration of bladder dysfunction in high-fat obese mice.
- Leiria LO, Sollon C, Calixto MC, Lintomen L, Mónica FZ, Anhê GF, De Nucci G, Zanesco A, Grant AD, Antunes E: Role of PKC and CaV1.2 in detrusor overactivity in a model of obesity associated with insulin resistance in mice. PLoS One. 2012, 7: e48507-10.1371/journal.pone.0048507.PubMed CentralView ArticlePubMedGoogle Scholar
- Leiria LO, Sollon C, Báu FR, Mónica FZ, D Ancona CL, De Nucci G, Grant AD, Anhê GF, Antunes E: Insulin relaxes human and mice bladder via PI3K/AKT/eNOS pathway activation in mucosal cells: UPR-dependent insulin resistance as a cause of obesity-associated overactive bladder. J Physiol. 2013, 591: 2259-2273.PubMed CentralView ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.