- Meeting abstract
- Open Access
Dephosphorylation of juxtamembrane serines and threonines of the NPR2 guanylyl cyclase regulates oocyte meiotic resumption
© Shuhaibar et al. 2015
- Published: 2 September 2015
- Luteinizing Hormone
- Granulosa Cell
- Cumulus Cell
- Antral Follicle
- Guanylyl Cyclase
To investigate whether the LH-induced decrease in guanylyl cyclase activity is required for meiotic resumption, we isolated Npr2-7E/7E and wild-type follicles and observed them in culture before and after addition of LH. In wild-type follicles, LH-induced nuclear envelope breakdown (NEBD) began at 2 hours and reached ~80% by 6 hours. In contrast, in Npr2-7E/7E follicles, no evidence of meiotic resumption was observed in the first 6 hours following treatment with LH. However, by 8 hours after LH application, NEBD had occurred in ~40% of Npr2-7E/7E follicle-enclosed oocytes, and by 12 hours NEBD was seen in ~80% of oocytes. During cumulus expansion preceding ovulation, gap junction communication between the oocyte and cumulus cells is disrupted . The LH-induced cumulus expansion occurred similarly in Npr2-7E/7E and wild-type follicles, suggesting that gap junction disruption during cumulus expansion might explain why meiosis eventually resumes in the NPR2-7E/7E follicles.
Dephosphorylation of NPR2 is required for the rapid resumption of meiosis in response to LH.
This work was supported by NIH grants R37HD014939, R01GM098309, T32AR050938 and the Fund for Science.
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