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  • Meeting abstract
  • Open Access

Dephosphorylation of juxtamembrane serines and threonines of the NPR2 guanylyl cyclase regulates oocyte meiotic resumption

  • 1,
  • 2,
  • 1,
  • 3,
  • 2 and
  • 1Email author
BMC Pharmacology and Toxicology201516 (Suppl 1) :A30

https://doi.org/10.1186/2050-6511-16-S1-A30

  • Published:

Keywords

  • Luteinizing Hormone
  • Granulosa Cell
  • Cumulus Cell
  • Antral Follicle
  • Guanylyl Cyclase

Background

The meiotic cell cycle of mammalian oocytes starts during embryogenesis and then pauses until luteinizing hormone (LH) restarts the cycle. This meiotic arrest is maintained by cGMP, which is produced in the granulosa cells by C-type natriuretic peptide (CNP) activation of NPR2 [1]. LH decreases cGMP in the granulosa cells, and via equilibration through gap junctions, cyclic GMP also decreases in the oocyte, thus releasing the meiotic arrest [2]. LH causes dephosphorylation and inactivation of NPR2 [3, 4], but whether NPR2 dephosphorylation is required for meiotic resumption is not known. Seven regulatory NPR2 phosphorylation sites have been identified (Fig. 1) [5, 6]. Here, we generated a knock-in mouse where each site was mutated to glutamate (Npr2-7E), resulting in a “constitutively phosphorylated” enzyme that we used to investigate the role of NPR2 dephosphorylation in the rapid resumption of meiosis in response to LH.
Figure 1
Figure 1

Diagram of NPR2 showing the seven serine and threonine phosphorylation sites that were changed to glutamate (E) in the Npr2 -7E mouse. The functional domains of the homodimeric transmembrane protein NPR2 are shown in gray. Binding of CNP (green) to the extracellular domain and phosphorylation of seven serine and threonine sites (red) is required for CNP-dependent activation of the guanylyl cyclase activity of NPR2. Dephosphorylation of these regulatory sites results in a decrease in guanylyl cyclase activity; the 7E mutation results in a protein that cannot be dephosphorylated.

Results

Membranes from isolated antral follicles treated with or without LH for 20 min were assayed for guanylyl cyclase activity under physiological conditions with CNP, ATP and Mg2+GTP (Fig. 2). In wild-type follicles treated with LH, the CNP-dependent guanylyl cyclase activity decreased to ~47% of initial values (Fig. 2, left). In contrast, LH caused no significant change in CNP-dependent guanylyl cyclase activity in Npr2-7E/7E follicle membranes (Fig. 2, right). Protein loss did not explain the reduction in CNP-dependent activity because activities measured with Mn2+GTP/Triton X-100 were not reduced by LH. These findings indicate that dephosphorylation of NPR2 is necessary for the hormonal regulation of guanylyl cyclase activity in the ovarian follicle.
Figure 2
Figure 2

Inhibition of the LH-induced decrease in guanylyl cyclase activity in mouse follicles by the Npr2 -7E mutations. Guanylyl cyclase activity in wild-type and Npr2-7E/7E follicle membranes, measured in the presence of 1 µM CNP. Membranes were prepared from follicles with or without treatment with LH for 20 min. Each value shows the mean ± s.e.m. for 4 separate membrane preparations. The asterisk indicates that LH treatment significantly decreased the CNP-dependent guanylyl cyclase activity in wild-type follicles (P<0.05). LH treatment did not cause a significant change in CNP-dependent activity in Npr2-7E/7E follicles (n.s.).

To investigate whether the LH-induced decrease in guanylyl cyclase activity is required for meiotic resumption, we isolated Npr2-7E/7E and wild-type follicles and observed them in culture before and after addition of LH. In wild-type follicles, LH-induced nuclear envelope breakdown (NEBD) began at 2 hours and reached ~80% by 6 hours. In contrast, in Npr2-7E/7E follicles, no evidence of meiotic resumption was observed in the first 6 hours following treatment with LH. However, by 8 hours after LH application, NEBD had occurred in ~40% of Npr2-7E/7E follicle-enclosed oocytes, and by 12 hours NEBD was seen in ~80% of oocytes. During cumulus expansion preceding ovulation, gap junction communication between the oocyte and cumulus cells is disrupted [7]. The LH-induced cumulus expansion occurred similarly in Npr2-7E/7E and wild-type follicles, suggesting that gap junction disruption during cumulus expansion might explain why meiosis eventually resumes in the NPR2-7E/7E follicles.

Conclusion

Dephosphorylation of NPR2 is required for the rapid resumption of meiosis in response to LH.

Declarations

Acknowledgements

This work was supported by NIH grants R37HD014939, R01GM098309, T32AR050938 and the Fund for Science.

Authors’ Affiliations

(1)
Department of Cell Biology, University of Connecticut Health Center, Farmington, USA
(2)
Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, USA
(3)
Department of Genetics and Genome Sciences, University of Connecticut Health Center, Farmington, USA

References

  1. Zhang M, Su Y-Q, Sugiura K, Xia G, Eppig JJ: Granulosa cell ligand NPPC and its receptor NPR2 maintain meiotic arrest in mouse oocytes. Science. 2010, 330: 366-369. 10.1126/science.1193573.View ArticlePubMedPubMed CentralGoogle Scholar
  2. Shuhaibar LC, Egbert JR, Norris RP, Lampe PD, Nikolaev VO, Thunemann M, Wen L, Feil R, Jaffe LA: Intercellular signaling via cyclic GMP diffusion through gap junctions in the mouse ovarian follicle. Proc. Natl. Acad. Sci. U. S. A. 2015, in pressGoogle Scholar
  3. Robinson JW, Zhang M, Shuhaibar LC, Norris RP, Geerts A, Wunder F, Eppig JJ, Potter LR, Jaffe LA: Luteinizing hormone reduces the activity of the NPR2 guanylyl cyclase in mouse ovarian follicles, contributing to the cyclic GMP decrease that promotes resumption of meiosis in oocytes. Dev Biol. 2012, 366: 308-316. 10.1016/j.ydbio.2012.04.019.View ArticlePubMedPubMed CentralGoogle Scholar
  4. Egbert JR, Shuhaibar LC, Edmund AB, Van Helden DA, Robinson JW, Uliasz TF, Baena V, Geerts A, Wunder F, Potter LR, Jaffe LA: Dephosphorylation and inactivation of the NPR2 guanylyl cyclase in the granulosa cells contributes to the LH-induced cGMP decrease that causes resumption of meiosis in rat oocytes. Development. 2014, 141: 3594-3604. 10.1242/dev.112219.View ArticlePubMedPubMed CentralGoogle Scholar
  5. Yoder AR, Stone MD, Griffin TJ, Potter LR: Mass spectrometric identification of phosphorylation sites in guanylyl cyclase A and B. Biochemistry. 2010, 49: 10137-10145. 10.1021/bi101700e.View ArticlePubMedPubMed CentralGoogle Scholar
  6. Yoder AR, Robinson JW, Dickey DM, Andersland J, Rose BA, Stone MD, Griffin TJ, Potter LR: A functional screen provides evidence for a conserved, regulatory, juxtamembrane phosphorylation site in guanylyl cyclase A and B. PLoS One. 2012, 7: e36747-10.1371/journal.pone.0036747.View ArticlePubMedPubMed CentralGoogle Scholar
  7. Gilula NB, Epstein ML, Beers WH: Cell-to-cell communication and ovulation. A study of the cumulus-oocyte complex. J Cell Biol. 1978, 78: 58-75. 10.1083/jcb.78.1.58.View ArticlePubMedGoogle Scholar

Copyright

© Shuhaibar et al. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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