Influence of smoking on vascular reactivity to cGMP generators in human internal thoracic arteries
© Tawa et al. 2015
Published: 2 September 2015
There are nitric oxide (NO)-sensitive and -insensitive forms of soluble guanylyl cyclase (sGC). This balance is shifted to the latter under stress conditions associated with increased production of reactive oxygen species (ROS) [1, 2]. The present study investigated whether smoking, a well-documented source of ROS, affects NO-sensitive and -insensitive sGC-mediated effects in human arteries.
Materials and methods
Mechanical responses of internal thoracic arteries obtained from 29 patients undergoing coronary artery bypass grafting were studied. The patients were divided into 2 groups according to their smoking habits: current or ever-smokers (n=17) and former or never-smokers (n=12). Concentration-response curves for nitroglycerin and BAY 60-2770 were constructed in helically-cut strips pre-contracted with phenylephrine, and the values of potency (pD2) were calculated. Relaxations induced by the agonists were presented as relative values to the relaxation caused by 10-4 M papaverine. Concentration-response curves were analyzed using two-way repeated measures analysis of variance and Bonferroni post hoc test. pD2 values were compared with unpaired two-tailed Student's t-test.
It was demonstrated that smoking is not causal factor for the imbalance between NO-sensitive and -insensitive forms of sGC in human internal thoracic arteries.
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