- Meeting abstract
- Open Access
Influence of smoking on vascular reactivity to cGMP generators in human internal thoracic arteries
© Tawa et al. 2015
- Published: 2 September 2015
- Reactive Oxygen Species
- Nitric Oxide
- Coronary Artery Bypass Grafting
There are nitric oxide (NO)-sensitive and -insensitive forms of soluble guanylyl cyclase (sGC). This balance is shifted to the latter under stress conditions associated with increased production of reactive oxygen species (ROS) [1, 2]. The present study investigated whether smoking, a well-documented source of ROS, affects NO-sensitive and -insensitive sGC-mediated effects in human arteries.
Mechanical responses of internal thoracic arteries obtained from 29 patients undergoing coronary artery bypass grafting were studied. The patients were divided into 2 groups according to their smoking habits: current or ever-smokers (n=17) and former or never-smokers (n=12). Concentration-response curves for nitroglycerin and BAY 60-2770 were constructed in helically-cut strips pre-contracted with phenylephrine, and the values of potency (pD2) were calculated. Relaxations induced by the agonists were presented as relative values to the relaxation caused by 10-4 M papaverine. Concentration-response curves were analyzed using two-way repeated measures analysis of variance and Bonferroni post hoc test. pD2 values were compared with unpaired two-tailed Student's t-test.
It was demonstrated that smoking is not causal factor for the imbalance between NO-sensitive and -insensitive forms of sGC in human internal thoracic arteries.
- Tawa M, Shimosato T, Iwasaki H, Imamura T, Okamura T: Effects of peroxynitrite on relaxation through the NO/sGC/cGMP pathway in isolated rat iliac arteries. J Vasc Res. 2014, 51 (6): 439-446. 10.1159/000371491.View ArticlePubMedGoogle Scholar
- Tawa M, Shimosato T, Iwasaki H, Imamura T, Okamura T: Different influences of extracellular and intracellular superoxide on relaxation through the NO/sGC/cGMP pathway in isolated rat iliac arteries. J Cardiovasc Pharmacol. 2015, 65 (2): 160-167.PubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.