- Meeting abstract
- Open Access
Structural basis of cyclic nucleotide selectivity in cGMP dependent protein kinase II
© Campbell et al. 2015
- Published: 2 September 2015
- Cystic Fibrosis
- Cyclic Nucleotide
- Nucleotide Binding Domain
- cGMP Dependent Protein Kinase
- Cyclic Nucleotide Binding
As a central mediator of the natriuretic peptide-cGMP signalling cascade, membrane bound type II cGMP dependent protein kinase (PKG II) is a key regulator of bone growth, renin secretion, and memory formation. It represents an important drug target for treating osteoporosis, cystic fibrosis, and memory loss [1–5]. In spite of its crucial physiological roles and its importance as a therapeutic target, little is known about its mechanisms of cyclic nucleotide selectivity and activation due to a lack of structural information. PKG II contains an N-terminal regulatory (R)-domain that binds a C-terminal catalytic (C)-domain in the absence of cGMP. Binding of cGMP to the cyclic nucleotide binding domains (CNB-A and B) within the R-domain releases the C-domain, leading to activation. We sought to understand the cyclic nucleotide selectivity and activation mechanisms of PKG II by studying each CNB domain.
We screened and identified CNB domains of PKG II that are suitable for our structural studies using a high throughput Ligation Independent Cloning method. Our affinity measurements of the resulting CNB domains showed that CNB-B binds cGMP with a higher affinity, providing almost 500-fold selectivity, while CNB-A only offers 10-fold selectivity . To understand the structural basis of each domain's cGMP selectivity, we solved crystal structures of CNB-A and -B in the presence of cyclic nucleotides. The structures revealed that only CNB-B shows an ordered C-helix that shields the cGMP pocket and specifically interacts with the guanine moiety through several hydrogen bond and VWD contacts. In contrast, CNB-A displays an open pocket without a C-terminal helix, resulting in fewer interactions with cGMP. Our mutation analysis demonstrated that the polar contacts at the C-helix of CNB-B are crucial for high cGMP selectivity and kinase activation.
- Leier G, Bangel-Ruland N, Sobczak K, Knieper Y, Weber WM: Sildenafil acts as potentiator and corrector of CFTR but might be not suitable for the treatment of CF lung disease. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology. 2012, 29: 775-790. 10.1159/000265129.View ArticleGoogle Scholar
- Pfeifer A, Aszodi A, Seidler U, Ruth P, Hofmann F, Fassler R: Intestinal secretory defects and dwarfism in mice lacking cGMP-dependent protein kinase II. Science. 1996, 274: 2082-2086. 10.1126/science.274.5295.2082.View ArticlePubMedGoogle Scholar
- Rangaswami H, Marathe N, Zhuang S, Chen Y, Yeh JC, Frangos JA, Boss GR, Pilz RB: Type II cGMP-dependent protein kinase mediates osteoblast mechanotransduction. J Biol Chem. 2009, 284: 14796-14808. 10.1074/jbc.M806486200.View ArticlePubMedPubMed CentralGoogle Scholar
- Vaandrager AB, Hogema BM, de Jonge HR: Molecular properties and biological functions of cGMP-dependent protein kinase II. Frontiers in bioscience: a journal and virtual library. 2005, 10: 2150-2164. 10.2741/1687.View ArticleGoogle Scholar
- Vaandrager AB, Smolenski A, Tilly BC, Houtsmuller AB, Ehlert EM, Bot AG, Edixhoven M, Boomaars WE, Lohmann SM, de Jonge HR: Membrane targeting of cGMP-dependent protein kinase is required for cystic fibrosis transmembrane conductance regulator Cl- channel activation. Proceedings of the National Academy of Sciences of the United States of America. 1998, 95: 1466-1471. 10.1073/pnas.95.4.1466.View ArticlePubMedPubMed CentralGoogle Scholar
- Huang GY, Kim JJ, Reger AS, Lorenz R, Moon EW, Zhao C, Casteel Darren E, Bertinetti D, VanSchouwen B, Selvaratnam R, Pflugrath JW, Sankaran B, Melacini G, Herberg FW, Kim C: Structural Basis for Cyclic-Nucleotide Selectivity and cGMP-Selective Activation of PKG I. Structure. 2014, 22: 116-124. 10.1016/j.str.2013.09.021.View ArticlePubMedGoogle Scholar
- Castro LR, Schittl J, Fischmeister R: Feedback control through cGMP-dependent protein kinase contributes to differential regulation and compartmentation of cGMP in rat cardiac myocytes. Circulation research. 2010, 107: 1232-1240. 10.1161/CIRCRESAHA.110.226712.View ArticlePubMedGoogle Scholar
- Conti M, Mika D, Richter W: Cyclic AMP compartments and signaling specificity: role of cyclic nucleotide phosphodiesterases. The Journal of general physiology. 2014, 143: 29-38.View ArticlePubMedPubMed CentralGoogle Scholar
- Neves SR, Tsokas P, Sarkar A, Grace EA, Rangamani P, Taubenfeld SM, Alberini CM, Schaff JC, Blitzer RD, Moraru II, Iyengar R: Cell shape and negative links in regulatory motifs together control spatial information flow in signaling networks. Cell. 2008, 133: 666-680. 10.1016/j.cell.2008.04.025.View ArticlePubMedPubMed CentralGoogle Scholar
- Wilson LS, Elbatarny HS, Crawley SW, Bennett BM, Maurice DH: Compartmentation and compartment-specific regulation of PDE5 by protein kinase G allows selective cGMP-mediated regulation of platelet functions. Proceedings of the National Academy of Sciences of the United States of America. 2008, 105: 13650-13655. 10.1073/pnas.0804738105.View ArticlePubMedPubMed CentralGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.