This article has Open Peer Review reports available.
Modeling of chemical inhibition from amyloid protein aggregation kinetics
© Vázquez; licensee BioMed Central Ltd. 2014
Received: 24 May 2013
Accepted: 14 February 2014
Published: 27 February 2014
The process of amyloid proteins aggregation causes several human neuropathologies. In some cases, e.g. fibrillar deposits of insulin, the problems are generated in the processes of production and purification of protein and in the pump devices or injectable preparations for diabetics. Experimental kinetics and adequate modelling of chemical inhibition from amyloid aggregation are of practical importance in order to study the viable processing, formulation and storage as well as to predict and optimize the best conditions to reduce the effect of protein nucleation.
In this manuscript, experimental data of insulin, Aβ42 amyloid protein and apomyoglobin fibrillation from recent bibliography were selected to evaluate the capability of a bivariate sigmoid equation to model them. The mathematical functions (logistic combined with Weibull equation) were used in reparameterized form and the effect of inhibitor concentrations on kinetic parameters from logistic equation were perfectly defined and explained. The surfaces of data were accurately described by proposed model and the presented analysis characterized the inhibitory influence on the protein aggregation by several chemicals. Discrimination between true and apparent inhibitors was also confirmed by the bivariate equation. EGCG for insulin (working at pH = 7.4/T = 37°C) and taiwaniaflavone for Aβ42 were the compounds studied that shown the greatest inhibition capacity.
An accurate, simple and effective model to investigate the inhibition of chemicals on amyloid protein aggregation has been developed. The equation could be useful for the clear quantification of inhibitor potential of chemicals and rigorous comparison among them.
The aggregation and fibrillation of proteins has been commonly associated with numerous degenerative disorders in humans including Alzheimer’s, Parkinson’s, prion’s, diabetes type II and Huntington’s diseases [1–5]. These proteins, called amylodogenics, are also involved in production, purification and formulation interferences of biotechnology and pharmacologic processes [6, 7]. The phenomenon of protein aggregation, led by nucleation-dependent polymerization mechanism, consists in the formation of amyloid fibrils formulated with β-sheet structures in cross-β-sheet arrangement producing insoluble aggregates [8–10]. For example, insulin peptide also generates fibrillar structures under specific conditions such as low pH, high temperatures or organic solvents dilution [11–13]. Those phase transformations are not observed in vivo but are very common, for example, in the clinical preparations of insulin for diabetics . Human amyloid proteins (Aβ) are peptides of rather 39-42 residues. Aβ40 contains 40 amino acids and Aβ42 is the major isoform in the Aβ peptides with 42 residues polypeptide chain and it is the responsible of amyloid plaques generated in Alzheimer’s disorder [15, 16].
In general, much of the success in the future application of chemicals to inhibit the in vivo formation of such fibrils is dependent on the correct modelling and evaluation of in vitro kinetic data in order to establish protocols of action, effectiveness of molecules and dose-optimization of compounds. The kinetic description of amyloid protein aggregation based on mechanistic and thermodynamic approaches have been extensively studied [17–22] and an ample number of plausible mechanisms of nucleation and fibrillation have been proposed [23, 24]. So remarkable, two new and similar proposal: “Ockham's razor”/minimalistic and Crystallization-like Model, have been recently developed. Both have solid biophysical basis and they were successfully applied to describe and explain the experimental data of different amyloid protein aggregation [25–28]. However, the combined effect of anti-aggregation protein agents, e.g. surfactants, osmolytes and food additives, on time-dependent responses has not been modelled by that way or by means of empirical equations.
Although the use of empirical sigmoid equations, mainly the logistic equation, does not provide a direct explanation of the molecular steps that underlie in the generation of fibrils, it is a robust tool to examine protein aggregation kinetic data and to address all the phases of the process [4, 29, 30]. In addition, other authors assimilated the parameters from logistic  to those obtained by an autocatalytic mechanism [23, 27]. Nonetheless, that equation is always formulated without the parameters (fibrillation rate and lag phase) in an explicit form hindering the estimation of their statistical error.
In the present work, the capability of fit and experimental data predictability of a sigmoid bivariate model that simulates the growth of aggregation process on different proteins along with the effects of inhibitory chemicals on the kinetic parameters is explored in selected cases obtained from the literature. The results reveal its efficacy and validity to analyze the most relevant parameters that describe geometrically and macroscopically the mentioned process.
Amyloid protein aggregation data were collected from results previously reported in the bibliography and digitized from the published curves using GetData Graph Digitizer 2.24. The kinetics of insulin inhibition induced by (−)-epigallocatechin-3-gallate (EGCG) were selected from Wang et al. , methylglyoxal effects were collected from Oliveira et al.  and those produced by 1,2-diheptanoyl-sn-glycero-3-phosphocholine (di-C7-PC) were described in Wang et al. . Two conditions of EGCG affecting to fibrillation kinetics were used: EGCG_1 (case 1) studied at pH = 2.0/T = 60°C and EGCG_2 (case 2) at pH = 7.4/T = 37°C.
On the other hand, the aggregation kinetics of Aβ42 amyloid protein inhibited by apigenin and taiwaniaflavone were selected from Thapa et al.  and the data affected by ectoine and hydroxyectoine from Kanapathipillai et al. . Finally, apomyoglobin fibrillation experiments were published in Vilasi et al. . The datasets were obtained by the two most common methods used to probe amyloid formation in vitro, the increment in light scattering of the protein solution due to insolubilization, and the increase in ThT fluorescence due to amyloid binding.
Symbolic notations used and corresponding units
Insulin aggregation kinetics measured by absorbance or fluorescence
Amyloid aggregation growth measured as absorbance at 600 nm, relative ThT fluorescence intensity (%) and ThT fluorescence intensity at 482 nm or 490 nm. Units: absorbance units (AU) or (%).
Time. Units: h or d
X m :
Maximum aggregation growth. Units: AU or %
v m :
Maximum aggregation rate. Units: AU h−1, AU d−1 or % h−1
Lag phase. Units: h or d
X m • :
Maximum insulin aggregation affected by chemical agent. Units: AU or %
v m • :
Maximum insulin aggregation rate affected by chemical agent. Units: AU h−1, AU d−1 or % h−1
Lag phase affected by chemical agent. Units: h or d
Concentration effects on insulin aggregation kinetics
Concentration of chemical agent. Units: mM or μM
K x :
Maximum response affecting on X m . Dimensionless
m x :
Concentration corresponding to the semi-maximum response affecting on X m . Units: mM or μM
a x :
Shape parameter affecting on X m . Dimensionless
K v :
Maximum response affecting on v m . Dimensionless
m v :
Concentration corresponding to the semi-maximum response affecting on v m . Units: mM or μM
a v :
Shape parameter affecting on v m . Dimensionless
K λ :
Maximum response affecting on λ. Dimensionless
m λ :
Concentration corresponding to the semi-maximum response affecting on λ. Units: mM or μM
a λ :
Shape parameter affecting on λ. Dimensionless
Additionally, a global parameter (EC 50,τ ) was also selected for the overall description of chemical effects on fibrillation growth studies according the algebraic steps previously described . This parameter was defined as the chemical concentration (in mM) that reduces the aggregation by 50% compared to that produced by the control without agent at time (τ) which also reduces the maximum aggregation by 50%.
Numerical methods and statistical analysis
The fitting procedures and parametric estimates from the experimental results were performed by minimizing the sum of quadratic differences between the observed and model-predicted values using the nonlinear least-squares (quasi-Newton) method provided by the ‘Solver’ macro from Microsoft Excel spreadsheet. The confidence intervals of the best-fit values for the parametric estimates (Student’s t test, α = 0.05), consistency of the mathematical models (Fisher’s F test; p < 0.05) and covariance and correlation matrices were calculated using the ‘SolverAid’ macro, which is freely available from Levie’s Excellaneous website http://www.bowdoin.edu/~rdelevie/exellaneous/. An example of the type of Excel spreadsheet used for modeling (Ab42amyloid apigenin case) was provided as Additional file 1. These statistical procedures and residual analysis (Durbin-Watson test) were confirmed and evaluated, respectively, by DataFit 9 (Oakdale Engineering, Oakdale, PA). Moreover, bias and accuracy factors of fittings (B f and A f , respectively) were calculated according to the expressions reported by Ross .
Results and discussion
Characteristics and simulations of bivariate model
In contrast, the fit using the reparameterized functions can be used to easily calculate the confidence intervals of the parameters. The algebraic steps required to obtain the corresponding reparameterization of λ and v m from the logistic equation are detailed in the Appendix. Thus, the parameters from this equation define all of the aggregation kinetic phases : pre-nucleation, which is characterized by λ, post-nucleation, which is represented by v m or τ, and elongation, which is determined by X m .
Arbitrary numerical values defined for the simulations of Figure 1 (A, B, C, D, E, F and G) according to the parameters defined in the equation ( 1)
Effect on X m
Effect on v m
Effect on λ
Inhibitory effect of EGCG on insulin aggregation
Parametric estimates and confidence intervals (α = 0.05) from the equation ( 1) applied to the aggregation insulin increased data influenced by EGCG_1, EGCG_2, di-C7-PC and methylglyoxal concentrations
X m (AU or %)
v m (AU h−1 or % h−1)
Effect on X m
m x (mM)
Effect on v m
m v (mM)
Effect on λ
m λ (mM)
EC 50,τ (mM)
R 2 adj
At an acidic pH and a high temperature, the effect of EGCG on the kinetic parameters was not significant for the maximum aggregation rate but statistically significant for X m and the lag phase. As can be observed in Figure 3A, the slopes of the sigmoid curves were parallel to the influence of the EGCG concentration, and the surface can be assimilated to that simulated in Figure 2D but with less effect on X m . These results show a longer pre-nucleation phase, which indicates that EGCG blocks the formation of the seeding nuclei without changing the fibril elongation rate (post-nucleation). This behaviour is consistent with the definition reported by Martins  who denominates the compounds that modify kinetic parameters (for instance in the present case, v m and/or λ) as true inhibitors.
In contrast, different surface and parametric responses were observed for EGCG_2 up to a concentration of 0.2 mM polyphenol with only significant modifications in the maximum aggregation growth parameter (Figure 3B). Under experimental conditions of higher pH and lower T, EGCG acts as an apparent inhibitor that only alters the thermodynamic properties of aggregation process . Higher concentrations of EGCG (from 0.35 to 0.7 mM) led to an increase in the fibrillation response more than the inhibition of the amyloid process . These experimental points were deleted in this mathematical analysis because biphasic aggregation data with profiles of decreasing and increasing values of X m cannot be fitted by the proposed equation or by any other simple equation.
The global description of chemical inhibition was calculated by means of a single index: EC 50, τ (Table 3). This parameter can be defined as a summary of all of the effects on the aggregation kinetics observed at the time required to reach the semi-maximum aggregation growth. Thus, the obtained value of this parameter can be used for the comparison and the evaluation of the application of chemicals to reduce the protein fibrillation. The results demonstrated that the most inhibitory conditions for EGCG are established at neutral pH and a lower temperature (case 2), but these conditions require a longer time (τ) to achieve the parameter EC 50, τ.
Inhibitory effect of di-C7-PC and methylglyoxal on insulin aggregation
Figure 3C displays the surface described by equation (1) and data on the insulin kinetics in the presence of a surfactant. The parameter values and the statistical characteristics of the modeling were also determined (Table 3). All of the statistical results revealed good agreement and accuracy between the observed and the predicted values, the consistency of the equation, and a lack of bias during the fitting process.
Regarding the effects, the two kinetic parameters v m and X m were significantly affected by di-C7-PC, whereas the lag phase was not modified by the surfactant (Student’s t test, α = 0.05), as determined in the simulated conditions defined by Figure 2B. This result is in agreement with data reported for different systems of effector/protein, such as copper/β-amyloid  or glutathione/hen egg-white lysozyme, although these data were obtained using a non-mathematical approach . The value of EC 50, τ was similar to that obtained for EGCG (case 1) for a lower initial concentration of insulin, but a much longer time was required to obtain this parametric value (150.68 h).
Similar inhibitory responses were also observed with methylglyoxal; thus, v m and X m were the kinetic parameters inhibited by this aldehyde form of pyruvic acid (Table 3 and Figure 3D). Under an initial insulin concentration (3 g/L) that was higher than the initial EGCG concentration, methylglyoxal effectively reduced the aggregation growth (0.33 mM) within a shorter time (3.9 h). Both chemicals (di-C7-PC and methylglyoxal), under the experimental conditions assayed, showed similar features of true inhibitors . The application of the proposed methodology may help the design of optimal strategies for the reduction of the fibril deposition of insulin in commercial preparations.
Inhibitory effect of mono and biflavonoids on Aβ42 amyloid protein
Parametric estimates and confidence intervals (α = 0.05) from the equation ( 1) applied to the aggregation of Aβ42-amyloid protein increased data influenced by apigenin, ectoine, hidroxyectoine and taiwaniaflavone concentrations
X m (AU)
(5.07±0.23) × 10−3
(4.96±0.24) × 10−3
v m (AU h−1 or AU d−1)
(0.24±0.05) × 10−3
(0.30±0.08) × 10−3
λ (h or d)
Effect on X m
m x (mM or μM)
Effect on v m
m v (mM or μM)
Effect on λ
m λ (mM or μM)
EC 50, τ (mM or μM)
τ (h or d)
R 2 adj
The numerical values of EC 50,τ confirmed the superior antifibrillogenic capacity of the biflavonoid taiwaniaflavone (EC 50,τ = 0.91 μM) compared with the monoflavonoid apigenin (EC 50,τ = 2.45 μM). However, the time τ was quite similar in both cases. This affirmation is consistent with the conclusions reported in the original work conducted by Thapa et al. .
Inhibitory effect of osmolytes on Aβ42 amyloid protein
Different types of molecules have been recently suggested as drug candidates for the treatment of neurodegenerative disorders caused by the anti-aggregation properties of amyloid proteins . One of the most interesting candidates are osmolytes (compounds that protect against the osmotic stress), such as the ectoines that are obtained from aerobic heterotrophic bacteria . The response surface for hydroxyectoine (Figure 4C) shows a behavior that is similar to that obtained with the simulation shown in Figure 2B. Based on the numerical fittings summarized in Table 4, it was statistically demonstrated that osmolytes inhibited the maximum aggregation rate and the maximum fibrillation growth. In contrast, no significant changes in the lag phase were observed. Therefore, both osmolytes effectively blocked the post-nucleation and the elongation stages but not the pre-nuclei formation. In addition, ectoine was much more effective as an antineurotoxic (EC 50,τ = 0.96 mM) than hydroxyectoine (EC 50,τ = 2.20 mM).
Inhibition of apomyoglobin fibrils formation by trehalose
The effect of trehalose on the apomyoglobin aggregation kinetics was also studied using model (1), and excellent statistical results were obtained from the modeling and the description of the experimental data (Figure 4D and Table 4). As in the previous case, the disaccharide significantly inhibited the two kinetic parameters v m and X m without affecting the pre-nucleation phase (λ). Nevertheless, its relative efficacy was obtained at a much higher concentration (21.43 mM), and much longer times (6.5 d) were required than compared with the other cases studied.
In summary, a general bivariate model that combines the logistic equation for the description of kinetics and the Weibull equation for the chemical-concentration response has been proposed for the characterization of the inhibitory effects produced by several chemicals on the growth of the aggregation of amyloid proteins. In all cases, the inhibitory effects on the kinetic parameters were established, and the theoretical response surfaces were in perfect agreement with the selected data. In addition, the recent definition of true and apparent inhibitors reported by Martins  can be also evaluated and validated. EGCG at pH 7.4 and a temperature of 37°C was the best option for the reduction of the formation of insulin fibrils (EC 50,τ = 6 μM) but the shortest time (τ) was obtained with methylglyoxal. For the Aβ42 amyloid protein, the biflavonoid taiwaniaflavone produced the highest inhibition response at the lowest dose (0.91 μM) and the shortest time (0.65 h). The bivariate equation was validated using data obtained by two methods based on different chemical phenomena. Although this model can not define the mechanisms of action of chemical inhibitors on protein aggregation, it provides a consistent tool for the comparison of the ability of such compounds in the inhibition of the protein fibrillation process, regardless of the method used for its determination, and is a first step for the optimization of the in vitro application of them. Further experiments and corresponding modeling should be done to establish its validity for in vivo applications of anti-aggregation chemicals.
Reparameterization of logistic equation
Weibull equation and bivariate model ( 1)
where, Y is the response and Y m the maximum response, m is the concentration corresponding to the semi-maximum response, C is the concentration and a is a shape parameter related to the maximum slope of the response.
When the equations (A.15) are inserting directly on equation (A.10) or (A.11), the bivariate model (1) is obtained.
The authors also thank the Unit of Information Resources for Research (URICI-CSIC) for the co-financing of this publication in Open Access.
- Bucciantini M, Giannoni E, Chiti F, Baroni F, Formigli L, Zurdo J, Taddei N, Ramponi G, Dobson CM, Stefani M: Inherent toxicity of aggregates implies a common mechanism for protein misfolding diseases. Nature. 2002, 416: 507-511.View ArticlePubMedGoogle Scholar
- Chiti F, Dobson CM: Protein misfolding, functional amyloid, and human disease. Annu Rev Biochem. 2006, 75: 333-366.View ArticlePubMedGoogle Scholar
- Stefani M, Dobson CM: Protein aggregation and aggregate toxicity: new insights into protein folding, misfolding diseases, and biological evolution. J Mol Med. 2003, 81: 678-699.View ArticlePubMedGoogle Scholar
- Uversky VN, Fink AL: Conformational constraints for amyloid fibrillation: the importance of being unfolded. Biochim Biophys Acta. 2004, 1698: 131-153.View ArticlePubMedGoogle Scholar
- Wang SSS, Good TA: An overview of Alzheimer's disease. J Chin Inst Chem Eng. 2005, 36: 533-559.Google Scholar
- Cromwell MEM, Hilario E, Jacobson F: Protein aggregation and bioprocessing. AAPS J. 2006, 8: E572-E579.View ArticlePubMedPubMed CentralGoogle Scholar
- Rosenberg AS: Effects of protein aggregates: an immunologic perspective. AAPS J. 2006, 8: E501-E507.View ArticlePubMedPubMed CentralGoogle Scholar
- Knowles TP, Fitzptrick AW, Meehan S, Mott HR, Vendruscolo M, Dobson CM, Welland ME: Role of intermolecular forces in defining material properties of protein nanofibrils. Science. 2007, 318: 1900-1903.View ArticlePubMedGoogle Scholar
- Nelson R, Eisenberg D: Recent atomic models of amyloid fibril structure. Curr Opin Struct Biol. 2006, 16: 260-265.View ArticlePubMedGoogle Scholar
- Ross CA, Poirier MA: What is the role of protein aggregation in neurodegeneration?. Nat Rev Mol Cell Biol. 2005, 6: 891-898.View ArticlePubMedGoogle Scholar
- Mauro M, Craparo EF, Podesta A, Bulone D, Carrotta R, Martorana V, Tiana G, San Biagio PL: Kinetics of different processes in human insulin amyloid formation. J Mol Biol. 2007, 366: 258-274.View ArticlePubMedGoogle Scholar
- Loksztejn A, Dzwolak W: Vortex-induced formation of insulin amyloid superstructures probed by time-lapse atomic force microscopy and circular dichroism spectroscopy. J Mol Biol. 2010, 395: 643-655.View ArticlePubMedGoogle Scholar
- Slusky V, Tamada JA, Klibanov AM, Langer R: Kinetics of insulin aggregation in aqueous solutions upon agitation in the presence of hydrophobic surfaces. Proc Natl Acad Sci U S A. 1991, 88: 9377-9381.View ArticleGoogle Scholar
- Dische FE, Wernstedt C, Westermark GT, Westermark P, Pepys MB, Rennie JA, Gilbey SG, Watkins PJ: Insulin as an amyloid-fibril protein at sites of repeated insulin injections in a diabetic patient. Diabetol. 1988, 31: 158-161.View ArticleGoogle Scholar
- Kanapathipillaia M, Lentzenb G, Sierksa M, Parka CB: Ectoine and hydroxyectoine inhibit aggregation and neurotoxicity of Alzheimer’s β-amyloid. FEBS Lett. 2005, 579: 4775-4780.View ArticleGoogle Scholar
- Thapa A, Woo ER, Chi EY, Sharoar MG, Jin HG, Shin SY, Park IS: Biflavonoids are superior to monoflavonoids in inhibiting amyloid-β toxicity and fibrillogenesis via accumulation of nontoxic oligomer-like structures. Biochem. 2011, 50: 2445-2455.View ArticleGoogle Scholar
- Andrews JM, Roberts CJ: A Lumry-Eyring nucleated polymerization model of protein aggregation kinetics: 1. Aggregation with pre-equilibrated unfolding. J Phys Chem B. 2007, 111: 7897-7913.View ArticlePubMedGoogle Scholar
- Bernacki J, Murphy RM: Model discrimination and mechanistic interpretation of kinetic data in protein aggregation studies. Biophys J. 2009, 96: 2871-2887.View ArticlePubMedPubMed CentralGoogle Scholar
- Ghosh P, Kumar A, Datta B, Rangacharim V: Dynamics of protofibril elongation and association involved in Aβ42 peptide aggregation in Alzheimer’s disease. BMC Bioinformatics. 2010, 11 (Suppl 6): S24-View ArticlePubMedPubMed CentralGoogle Scholar
- Lee CC, Nayak A, Sethuraman A, Belfort G, McRae GJ: A three-stage kinetic model of amyloid fibrillation. Biophys J. 2007, 92: 3448-3458.View ArticlePubMedPubMed CentralGoogle Scholar
- Roberts CJ: Kinetics of irreversible protein aggregation: Analysis of extended Lumry-Eyring models and implications for predicting protein shelf life. J Phys Chem B. 2003, 107: 1194-1207.View ArticleGoogle Scholar
- Ruzafa D, Conejero-Lara F, Morel B: Modulation of the stability of amyloidogenic precursors by anion binding strongly influences the rate of amyloid nucleation. Phys Chem Chem Phys. 2013, 15: 15508-15517.View ArticlePubMedGoogle Scholar
- Ruzafa D, Morel B, Varela L, Azuaga AI, Conejero-Lara F: Characterization of oligomers of heterogeneous size as precursors of amyloid fibril nucleation of an SH3 domain: an experimental kinetics study. Plos One. 2012, 7: e49690-View ArticlePubMedPubMed CentralGoogle Scholar
- Morris AM, Watzky MA, Finke RG: Protein aggregation kinetics, mechanism, and curve-fitting: a review of the literature. Biochim Biophys Acta. 2009, 1794: 375-397.View ArticlePubMedGoogle Scholar
- Crespo R, Rocha FA, Damas AM, Martins PM: A generic crystallization-like model that describes the kinetics of amyloid fibril formation. J Biol Chem. 2012, 287: 30585-30594.View ArticlePubMedPubMed CentralGoogle Scholar
- Martins PM: True and apparent inhibition of amyloid fibril formation. Prion. 2013, 7: 136-139.View ArticlePubMedPubMed CentralGoogle Scholar
- Morris AM, Watzky MA, Agar JN, Finke RG: Fitting neurological protein aggregation kinetic data via a 2-step, minimal/“Ockham’s Razor” model: the Finke-Watzky mechanism of nucleation followed by autocatalytic surface growth. Biochem. 2008, 47: 2413-2427.View ArticleGoogle Scholar
- Morris AM, Finke RG: α-Synuclein aggregation variable temperature and variable pH kinetic data: a re-analysis using the Finke–Watzky 2-step model of nucleation and autocatalytic growth. Biophys Chem. 2009, 140: 9-15.View ArticlePubMedGoogle Scholar
- Naiki H, Hasegawa K, Yamaguchi I, Nakamura H, Gejyo F, Nakakuki K: Apolipoprotein E and antioxidants have different mechanisms of inhibiting Alzheimer’s β-Amyloid fibril formation in vitro. Biochem. 1998, 37: 17882-17889.View ArticleGoogle Scholar
- Nielsen L, Khurana R, Coats A, Frokjaer S, Brange J, Vyas S, Uversky VN, Fink AL: Effect of environmental factors on the kinetics of insulin fibril formation: elucidation of the molecular mechanism. Biochem. 2001, 40: 6036-6046.View ArticleGoogle Scholar
- Wang SH, Dong XY, Sun Y: Effect of (−)-epigallocatechin-3-gallate on human insulin fibrillation/aggregation kinetics. Biochem Eng J. 2012, 63: 38-49.View ArticleGoogle Scholar
- Oliveira LMA, Lages A, Gomes RA, Neves H, Família C, Coelho AV, Quintas A: Insulin glycation by methylglyoxal results in native-like aggregation and inhibition of fibril formation. BMC Biochem. 2011, 12: 41-View ArticlePubMedPubMed CentralGoogle Scholar
- Wang SSS, Liu KN, Han TC: Amyloid fibrillation and cytotoxicity of insulin are inhibited by the amphiphilic surfactants. Biochim Biophys Acta. 2010, 1802: 519-530.View ArticlePubMedGoogle Scholar
- Vilasi S, Iannuzzi C, Portaccio M, Irace G, Sirangelo I: Effect of trehalose on W7FW14F apomyoglobin and insulin fibrillization: new insight into inhibition activity. Biochem. 2008, 47: 1789-1796.View ArticleGoogle Scholar
- Murado MA, Vázquez JA, Rial D, Beiras R: Dose-response modeling with two agents: application to the bioassay of oil and shoreline cleaning agents. J Hazardous Materials. 2011, 185: 807-817.View ArticleGoogle Scholar
- Riobó P, Paz B, Franco JM, Vázquez JA, Murado MA, Cacho E: Mouse bioassay for palytoxin. Specific symptoms and dose-response against dose-death time relationships. Food Chem Toxicol. 2008, 46: 2639-2647.View ArticlePubMedGoogle Scholar
- Vázquez JA, Murado MA: Mathematical tools for objective comparison of microbial cultures Application to evaluation of 15 peptones for lactic acid bacteria productions. Biochem Eng J. 2008, 39: 276-287.View ArticleGoogle Scholar
- Rial D, Vázquez JA, Murado MA: Effects of three heavy metals on the bacteria growth kinetics: a bivariate model for toxicological assessment. Appl Microbiol Biotechnol. 2011, 90: 1095-1109.View ArticlePubMedGoogle Scholar
- Vázquez JA, Durán A, Rodríguez-Amado I, Prieto MA, Rial D, Murado MA: Evaluation of toxic effects of several carboxylic acids on bacterial growth by toxicodynamic modelling. Microb Cell Fact. 2011, 10: 100-View ArticlePubMedPubMed CentralGoogle Scholar
- Ross T: Indices for performance evaluation of predictive models in food microbiology. J Appl Bacteriol. 1996, 81: 501-508.PubMedGoogle Scholar
- Vázquez JA, Murado MA: Unstructured mathematical model for biomass, lactic acid and bacteriocin production by lactic acid bacteria in batch fermentation. J Chem Technol Biotechnol. 2008, 83: 91-96.View ArticleGoogle Scholar
- Sabaté R, Villar-Piqué A, Espargaró A, Ventura S: Temperature dependence of the aggregation kinetics of Sup35 and Ure2p yeast prions. Biomacromolecules. 2012, 13: 474-483.View ArticlePubMedGoogle Scholar
- Fändrich M: Absolute correlation between lag time and growth rate in the spontaneous formation of several amyloid-like aggregates and fibrils. J Mol Biol. 2007, 365: 1266-1270.View ArticlePubMedGoogle Scholar
- Vázquez JA, Lorenzo JM, Fuciños P, Franco D: Evaluation of non-linear equations to model different animal growths with mono and bisigmoid profiles. J Theor Biol. 2012, 314: 95-105.View ArticlePubMedGoogle Scholar
- Murado MA, González MP, Vázquez JA: Dose-reponse relationships. An overview a generative model and its application to the verification of descriptive models. Enz Microb Technol. 2002, 31: 439-455.View ArticleGoogle Scholar
- Riobó P, Paz B, Franco JM, Vázquez JA, Murado MA: Proposal for a simple and sensitive haemolytic assay for palytoxin. Toxicological dynamics, kinetics, ouabain inhibition and thermal stability. Harmful Algae. 2008, 7: 415-429.View ArticleGoogle Scholar
- Shimanouchi T, Onishi R, Kitaura N, Umakoshi H, Kuboi R: Copper-mediated growth of amyloid β fibrils in the presence of oxidized and negatively charged liposomes. J Biosc Bioeng. 2011, 112: 611-615.View ArticleGoogle Scholar
- Wang SSS, Chou SW, Liu KN, Wu CH: Effects of glutathione on amyloid fibrillation of hen egg-white lysozyme. Int J Biol Macromol. 2009, 45: 321-329.View ArticlePubMedGoogle Scholar
- Arora A, Ha C, Park CB: Inhibition of insulin amyloid formation by small stress molecules. FEBS Lett. 2004, 564: 121-125.View ArticlePubMedGoogle Scholar
- Vorob’eva LI: Stressors, stress reactions, and survival of bacteria: a review. Appl Biochem Microbiol. 2004, 40: 261-269.View ArticleGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/2050-6511/15/9/prepub
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.